Salicylic Acid and Jasmonic Acid Increase the Polysaccharide Production of <i>Nostoc flagelliforme</i> via the Regulation of the Intracellular NO Level
To significantly improve the polysaccharide production of <i>Nostoc flagelliforme</i>, a total of 12 chemicals were evaluated for their effects on polysaccharide accumulation. The results showed that salicylic acid and jasmonic acid increased the accumulation of the polysaccharides in &l...
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MDPI AG
2023-02-01
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| Online Access: | https://www.mdpi.com/2304-8158/12/5/915 |
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| author | Cheng-Feng Han Shu-Ting Liu Rong-Rong Yan Jian Li Ni Chen Le-Le Zhang Shi-Ru Jia Pei-Pei Han |
| author_facet | Cheng-Feng Han Shu-Ting Liu Rong-Rong Yan Jian Li Ni Chen Le-Le Zhang Shi-Ru Jia Pei-Pei Han |
| author_sort | Cheng-Feng Han |
| collection | DOAJ |
| description | To significantly improve the polysaccharide production of <i>Nostoc flagelliforme</i>, a total of 12 chemicals were evaluated for their effects on polysaccharide accumulation. The results showed that salicylic acid and jasmonic acid increased the accumulation of the polysaccharides in <i>N. flagelliforme</i> significantly, by more than 20%. Three polysaccharides, namely control-capsule polysaccharide, salicylic acid-capsule polysaccharide, and jasmonic acid-capsule polysaccharide, were extracted and purified from <i>N. flagelliforme</i> under normal, salicylic acid, and jasmonic acid culture conditions, respectively. Their chemical compositions slightly differed regarding the total sugar and uronic acid contents, with average molecular weights of 2.06 × 10<sup>3</sup>, 2.16 × 10<sup>3</sup> and 2.04 × 10<sup>3</sup> kDa, respectively. They presented similar Fourier transform infrared spectra and no significant difference in antioxidant activity. It was revealed that the salicylic acid and jasmonic acid significantly increased the level of nitric oxide. By investigating the effects of the exogenous nitric oxide scavenger and nitric oxide donor on the nitric oxide levels and polysaccharide yield of <i>N. flagelliforme</i>, the results showed that the increase in intracellular nitric oxide levels might be an important factor promoting the accumulation of polysaccharides. These findings provide a theoretical foundation for enhancing the yield of secondary metabolites by regulating the intracellular nitric oxide levels. |
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| series | Foods |
| spelling | doaj.art-f3345e40d2104d0cacafed1df84dd94c2023-11-17T07:39:41ZengMDPI AGFoods2304-81582023-02-0112591510.3390/foods12050915Salicylic Acid and Jasmonic Acid Increase the Polysaccharide Production of <i>Nostoc flagelliforme</i> via the Regulation of the Intracellular NO LevelCheng-Feng Han0Shu-Ting Liu1Rong-Rong Yan2Jian Li3Ni Chen4Le-Le Zhang5Shi-Ru Jia6Pei-Pei Han7State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, ChinaTo significantly improve the polysaccharide production of <i>Nostoc flagelliforme</i>, a total of 12 chemicals were evaluated for their effects on polysaccharide accumulation. The results showed that salicylic acid and jasmonic acid increased the accumulation of the polysaccharides in <i>N. flagelliforme</i> significantly, by more than 20%. Three polysaccharides, namely control-capsule polysaccharide, salicylic acid-capsule polysaccharide, and jasmonic acid-capsule polysaccharide, were extracted and purified from <i>N. flagelliforme</i> under normal, salicylic acid, and jasmonic acid culture conditions, respectively. Their chemical compositions slightly differed regarding the total sugar and uronic acid contents, with average molecular weights of 2.06 × 10<sup>3</sup>, 2.16 × 10<sup>3</sup> and 2.04 × 10<sup>3</sup> kDa, respectively. They presented similar Fourier transform infrared spectra and no significant difference in antioxidant activity. It was revealed that the salicylic acid and jasmonic acid significantly increased the level of nitric oxide. By investigating the effects of the exogenous nitric oxide scavenger and nitric oxide donor on the nitric oxide levels and polysaccharide yield of <i>N. flagelliforme</i>, the results showed that the increase in intracellular nitric oxide levels might be an important factor promoting the accumulation of polysaccharides. These findings provide a theoretical foundation for enhancing the yield of secondary metabolites by regulating the intracellular nitric oxide levels.https://www.mdpi.com/2304-8158/12/5/915salicylic acidjasmonic acid<i>Nostoc flagelliforme</i> polysaccharidesnitric oxide levelsphysicochemical properties |
| spellingShingle | Cheng-Feng Han Shu-Ting Liu Rong-Rong Yan Jian Li Ni Chen Le-Le Zhang Shi-Ru Jia Pei-Pei Han Salicylic Acid and Jasmonic Acid Increase the Polysaccharide Production of <i>Nostoc flagelliforme</i> via the Regulation of the Intracellular NO Level Foods salicylic acid jasmonic acid <i>Nostoc flagelliforme</i> polysaccharides nitric oxide levels physicochemical properties |
| title | Salicylic Acid and Jasmonic Acid Increase the Polysaccharide Production of <i>Nostoc flagelliforme</i> via the Regulation of the Intracellular NO Level |
| title_full | Salicylic Acid and Jasmonic Acid Increase the Polysaccharide Production of <i>Nostoc flagelliforme</i> via the Regulation of the Intracellular NO Level |
| title_fullStr | Salicylic Acid and Jasmonic Acid Increase the Polysaccharide Production of <i>Nostoc flagelliforme</i> via the Regulation of the Intracellular NO Level |
| title_full_unstemmed | Salicylic Acid and Jasmonic Acid Increase the Polysaccharide Production of <i>Nostoc flagelliforme</i> via the Regulation of the Intracellular NO Level |
| title_short | Salicylic Acid and Jasmonic Acid Increase the Polysaccharide Production of <i>Nostoc flagelliforme</i> via the Regulation of the Intracellular NO Level |
| title_sort | salicylic acid and jasmonic acid increase the polysaccharide production of i nostoc flagelliforme i via the regulation of the intracellular no level |
| topic | salicylic acid jasmonic acid <i>Nostoc flagelliforme</i> polysaccharides nitric oxide levels physicochemical properties |
| url | https://www.mdpi.com/2304-8158/12/5/915 |
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