Bioelectrochemistry of nucleic acids for early cancer diagnostics – analysis of DNA methylation and detection of microRNAs
Dysregulation of gene expression mechanisms has been observed in many tumors, making their analysis of utmost importance. These mechanisms include DNA methylation, an epigenetic mechanism in which 5-carbon of cytosine becomes methylated, leading to gene silencing, and action of short RNA molecules c...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2017-03-01
|
| Series: | Reviews in Analytical Chemistry |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/revac-2016-0022 |
| _version_ | 1829142696360411136 |
|---|---|
| author | Bartosik Martin Hrstka Roman |
| author_facet | Bartosik Martin Hrstka Roman |
| author_sort | Bartosik Martin |
| collection | DOAJ |
| description | Dysregulation of gene expression mechanisms has been observed in many tumors, making their analysis of utmost importance. These mechanisms include DNA methylation, an epigenetic mechanism in which 5-carbon of cytosine becomes methylated, leading to gene silencing, and action of short RNA molecules called microRNAs, which regulate protein synthesis at post-transcriptional level by binding to mRNAs. In this review, we describe major roles of both mechanisms in carcinogenesis, offer an overview of currently used methods for their analysis, and summarize most recent advances in electrochemical-based assays and strategies. Advantages of electrochemistry, including favorable cost, time of experiment, or simple instrumentation, are highlighted, along with current challenges that need to be addressed prior to successful application into clinical routine. |
| first_indexed | 2024-12-14T20:36:50Z |
| format | Article |
| id | doaj.art-f3437351eb86453cbf8b2bd5ac2a6296 |
| institution | Directory Open Access Journal |
| issn | 0793-0135 2191-0189 |
| language | English |
| last_indexed | 2024-12-14T20:36:50Z |
| publishDate | 2017-03-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Reviews in Analytical Chemistry |
| spelling | doaj.art-f3437351eb86453cbf8b2bd5ac2a62962022-12-21T22:48:23ZengDe GruyterReviews in Analytical Chemistry0793-01352191-01892017-03-0136121522010.1515/revac-2016-0022Bioelectrochemistry of nucleic acids for early cancer diagnostics – analysis of DNA methylation and detection of microRNAsBartosik Martin0Hrstka Roman1Regional Centre for Applied Molecular Oncology (RECAMO), Masaryk Memorial Cancer Institute, Zluty kopec 7, 656 53 Brno, Czech RepublicRegional Centre for Applied Molecular Oncology (RECAMO), Masaryk Memorial Cancer Institute, Zluty kopec 7, 656 53 Brno, Czech RepublicDysregulation of gene expression mechanisms has been observed in many tumors, making their analysis of utmost importance. These mechanisms include DNA methylation, an epigenetic mechanism in which 5-carbon of cytosine becomes methylated, leading to gene silencing, and action of short RNA molecules called microRNAs, which regulate protein synthesis at post-transcriptional level by binding to mRNAs. In this review, we describe major roles of both mechanisms in carcinogenesis, offer an overview of currently used methods for their analysis, and summarize most recent advances in electrochemical-based assays and strategies. Advantages of electrochemistry, including favorable cost, time of experiment, or simple instrumentation, are highlighted, along with current challenges that need to be addressed prior to successful application into clinical routine.https://doi.org/10.1515/revac-2016-0022cancer diagnosticsdna methylationelectrochemistrymicrornamolecular oncology |
| spellingShingle | Bartosik Martin Hrstka Roman Bioelectrochemistry of nucleic acids for early cancer diagnostics – analysis of DNA methylation and detection of microRNAs Reviews in Analytical Chemistry cancer diagnostics dna methylation electrochemistry microrna molecular oncology |
| title | Bioelectrochemistry of nucleic acids for early cancer diagnostics – analysis of DNA methylation and detection of microRNAs |
| title_full | Bioelectrochemistry of nucleic acids for early cancer diagnostics – analysis of DNA methylation and detection of microRNAs |
| title_fullStr | Bioelectrochemistry of nucleic acids for early cancer diagnostics – analysis of DNA methylation and detection of microRNAs |
| title_full_unstemmed | Bioelectrochemistry of nucleic acids for early cancer diagnostics – analysis of DNA methylation and detection of microRNAs |
| title_short | Bioelectrochemistry of nucleic acids for early cancer diagnostics – analysis of DNA methylation and detection of microRNAs |
| title_sort | bioelectrochemistry of nucleic acids for early cancer diagnostics analysis of dna methylation and detection of micrornas |
| topic | cancer diagnostics dna methylation electrochemistry microrna molecular oncology |
| url | https://doi.org/10.1515/revac-2016-0022 |
| work_keys_str_mv | AT bartosikmartin bioelectrochemistryofnucleicacidsforearlycancerdiagnosticsanalysisofdnamethylationanddetectionofmicrornas AT hrstkaroman bioelectrochemistryofnucleicacidsforearlycancerdiagnosticsanalysisofdnamethylationanddetectionofmicrornas |