Purification of phospholipase C from Bacillus cereus by hydrophobic chromatography on palmitoyl cellulose.
Phospholipase C (phosphatidylcholine choline-phosphohydrolase, EC 3.1.4.E) from Bacillus cereus (IAM-1208) was adsorbed to palmitoyl cellulose from a crude enzyme solution at pH 5–9. The adsorption was not influenced by ionic strength up to 2 M NaCl. The adsorbed enzyme was eluted almost completely...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1979-05-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520406078 |