Development of a touchdown droplet digital PCR assay for the detection and quantitation of human papillomavirus 16 and 18 from self-collected anal samples
ABSTRACT Broad-spectrum droplet digital polymerase chain reaction (ddPCR) assays can be used to quantitate multiple human papillomavirus (HPV) types. However, the presence of nucleotide mismatches at the primer binding sites may result in different optimal annealing temperatures and, consequently, u...
Main Authors: | , , , , , , , |
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Format: | Article |
Jezik: | English |
Izdano: |
American Society for Microbiology
2023-12-01
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Serija: | Microbiology Spectrum |
Teme: | |
Online dostop: | https://journals.asm.org/doi/10.1128/spectrum.01836-23 |