Development of a touchdown droplet digital PCR assay for the detection and quantitation of human papillomavirus 16 and 18 from self-collected anal samples

ABSTRACT Broad-spectrum droplet digital polymerase chain reaction (ddPCR) assays can be used to quantitate multiple human papillomavirus (HPV) types. However, the presence of nucleotide mismatches at the primer binding sites may result in different optimal annealing temperatures and, consequently, u...

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Bibliografske podrobnosti
Main Authors: Gholamreza Haqshenas, Suzanne M. Garland, Prisha Balgovind, Alyssa Cornall, Jennifer Danielewski, Monica Molano, Dorothy A. Machalek, Gerald Murray
Format: Article
Jezik:English
Izdano: American Society for Microbiology 2023-12-01
Serija:Microbiology Spectrum
Teme:
Online dostop:https://journals.asm.org/doi/10.1128/spectrum.01836-23