Fluorescence of dyes in solutions with high absorbance. Inner filter effect correction.

Fluorescence is a proven tool in all fields of knowledge, including biology and medicine. A significant obstacle in its use is the nonlinearity of the dependence of the fluorescence intensity on fluorophore concentration that is caused by the so-called primary inner filter effect. The existing metho...

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Bibliographic Details
Main Authors: Alexander V Fonin, Anna I Sulatskaya, Irina M Kuznetsova, Konstantin K Turoverov
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4114876?pdf=render