Cellular heterogeneity in the 16HBE14o− airway epithelial line impacts biological readouts
Abstract The airway epithelial cell line, 16HBE14o−, is an important cell model for studying airway disease. 16HBE14o− cells were originally generated from primary human bronchial epithelial cells by SV40‐mediated immortalization, a process that is associated with genomic instability through long‐te...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-06-01
|
| Series: | Physiological Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.14814/phy2.15700 |
| _version_ | 1797397177925894144 |
|---|---|
| author | Jenny L. Kerschner Alekh Paranjapye Ann Harris |
| author_facet | Jenny L. Kerschner Alekh Paranjapye Ann Harris |
| author_sort | Jenny L. Kerschner |
| collection | DOAJ |
| description | Abstract The airway epithelial cell line, 16HBE14o−, is an important cell model for studying airway disease. 16HBE14o− cells were originally generated from primary human bronchial epithelial cells by SV40‐mediated immortalization, a process that is associated with genomic instability through long‐term culture. Here, we explore the heterogeneity of these cells, with respect to expression of the cystic fibrosis transmembrane conductance regulator (CFTR) transcript and protein. We isolate clones of 16HBE14o− with stably higher and lower levels of CFTR in comparison to bulk 16HBE14o−, designated CFTRhigh and CFTRlow. Detailed characterization of the CFTR locus in these clones by ATAC‐seq and 4C‐seq showed open chromatin profiles and higher order chromatin structure that correlate with CFTR expression levels. Transcriptomic profiling of CFTRhigh and CFTRlow cells showed that the CFTRhigh cells had an elevated inflammatory/innate immune response phenotype. These results encourage caution in interpreting functional data from clonal lines of 16HBE14o− cells, generated after genomic or other manipulations. |
| first_indexed | 2024-03-09T01:06:13Z |
| format | Article |
| id | doaj.art-f3b80707068b4937ab72b9f4a67a4357 |
| institution | Directory Open Access Journal |
| issn | 2051-817X |
| language | English |
| last_indexed | 2024-03-09T01:06:13Z |
| publishDate | 2023-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj.art-f3b80707068b4937ab72b9f4a67a43572023-12-11T09:37:12ZengWileyPhysiological Reports2051-817X2023-06-011111n/an/a10.14814/phy2.15700Cellular heterogeneity in the 16HBE14o− airway epithelial line impacts biological readoutsJenny L. Kerschner0Alekh Paranjapye1Ann Harris2Department of Genetics and Genome Sciences Case Western Reserve University Cleveland Ohio USADepartment of Genetics and Genome Sciences Case Western Reserve University Cleveland Ohio USADepartment of Genetics and Genome Sciences Case Western Reserve University Cleveland Ohio USAAbstract The airway epithelial cell line, 16HBE14o−, is an important cell model for studying airway disease. 16HBE14o− cells were originally generated from primary human bronchial epithelial cells by SV40‐mediated immortalization, a process that is associated with genomic instability through long‐term culture. Here, we explore the heterogeneity of these cells, with respect to expression of the cystic fibrosis transmembrane conductance regulator (CFTR) transcript and protein. We isolate clones of 16HBE14o− with stably higher and lower levels of CFTR in comparison to bulk 16HBE14o−, designated CFTRhigh and CFTRlow. Detailed characterization of the CFTR locus in these clones by ATAC‐seq and 4C‐seq showed open chromatin profiles and higher order chromatin structure that correlate with CFTR expression levels. Transcriptomic profiling of CFTRhigh and CFTRlow cells showed that the CFTRhigh cells had an elevated inflammatory/innate immune response phenotype. These results encourage caution in interpreting functional data from clonal lines of 16HBE14o− cells, generated after genomic or other manipulations.https://doi.org/10.14814/phy2.1570016HBE14o− cell linecellular heterogeneityCFTRtranscriptome |
| spellingShingle | Jenny L. Kerschner Alekh Paranjapye Ann Harris Cellular heterogeneity in the 16HBE14o− airway epithelial line impacts biological readouts Physiological Reports 16HBE14o− cell line cellular heterogeneity CFTR transcriptome |
| title | Cellular heterogeneity in the 16HBE14o− airway epithelial line impacts biological readouts |
| title_full | Cellular heterogeneity in the 16HBE14o− airway epithelial line impacts biological readouts |
| title_fullStr | Cellular heterogeneity in the 16HBE14o− airway epithelial line impacts biological readouts |
| title_full_unstemmed | Cellular heterogeneity in the 16HBE14o− airway epithelial line impacts biological readouts |
| title_short | Cellular heterogeneity in the 16HBE14o− airway epithelial line impacts biological readouts |
| title_sort | cellular heterogeneity in the 16hbe14o airway epithelial line impacts biological readouts |
| topic | 16HBE14o− cell line cellular heterogeneity CFTR transcriptome |
| url | https://doi.org/10.14814/phy2.15700 |
| work_keys_str_mv | AT jennylkerschner cellularheterogeneityinthe16hbe14oairwayepitheliallineimpactsbiologicalreadouts AT alekhparanjapye cellularheterogeneityinthe16hbe14oairwayepitheliallineimpactsbiologicalreadouts AT annharris cellularheterogeneityinthe16hbe14oairwayepitheliallineimpactsbiologicalreadouts |