pBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination

pBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination

Streptococcus pyogenes, or Group A Streptococcus (GAS), is a major human pathogen for which genetic manipulation remains an ongoing challenge. We created a new temperature-sensitive plasmid pBFK expressing spectinomycin resistance adapted to homologous recombination procedure to perform a complete g...

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Bibliographic Details
Main Authors: S. Boukthir, P. Gaudu, A. Faili, S. Kayal
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:Heliyon
Subjects:
Streptococcus pyogenes
Group A Streptococcus
Homologous recombination
Gene deletion
Isogenic mutant
Online Access:http://www.sciencedirect.com/science/article/pii/S2405844023039270

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http://www.sciencedirect.com/science/article/pii/S2405844023039270

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