pBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination
Streptococcus pyogenes, or Group A Streptococcus (GAS), is a major human pathogen for which genetic manipulation remains an ongoing challenge. We created a new temperature-sensitive plasmid pBFK expressing spectinomycin resistance adapted to homologous recombination procedure to perform a complete g...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023039270 |
| _version_ | 1827931841896644608 |
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| author | S. Boukthir P. Gaudu A. Faili S. Kayal |
| author_facet | S. Boukthir P. Gaudu A. Faili S. Kayal |
| author_sort | S. Boukthir |
| collection | DOAJ |
| description | Streptococcus pyogenes, or Group A Streptococcus (GAS), is a major human pathogen for which genetic manipulation remains an ongoing challenge. We created a new temperature-sensitive plasmid pBFK expressing spectinomycin resistance adapted to homologous recombination procedure to perform a complete gene deletion in GAS. Herein the mutagenesis strategy with pBFK was performed in a highly virulent GAS emm3 genotype. |
| first_indexed | 2024-03-13T06:59:38Z |
| format | Article |
| id | doaj.art-f3cd8c956b9548c38a15205a5af622f9 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-13T06:59:38Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-f3cd8c956b9548c38a15205a5af622f92023-06-07T04:49:12ZengElsevierHeliyon2405-84402023-06-0196e16720pBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombinationS. Boukthir0P. Gaudu1A. Faili2S. Kayal3CHU de Rennes, Service de Bactériologie-Hygiène Hospitalière, Rennes, France; Inserm, CIC 1414, Rennes, France; Corresponding author. CHU de Rennes, Service de Bactériologie-Hygiène Hospitalière, Rennes, France.Micalis Institute, INRAE, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, FranceInserm, CIC 1414, Rennes, France; OSS- Oncogenesis, Stress, Signaling, INSERM 1242, Rennes, France; Université de Rennes, Faculté de Pharmacie, Rennes, FranceCHU de Rennes, Service de Bactériologie-Hygiène Hospitalière, Rennes, France; Inserm, CIC 1414, Rennes, France; OSS- Oncogenesis, Stress, Signaling, INSERM 1242, Rennes, France; Université de Rennes, Faculté de Médecine, Rennes, France; Corresponding author. CHU de Rennes, Service de Bactériologie-Hygiène Hospitalière, Rennes, France.Streptococcus pyogenes, or Group A Streptococcus (GAS), is a major human pathogen for which genetic manipulation remains an ongoing challenge. We created a new temperature-sensitive plasmid pBFK expressing spectinomycin resistance adapted to homologous recombination procedure to perform a complete gene deletion in GAS. Herein the mutagenesis strategy with pBFK was performed in a highly virulent GAS emm3 genotype.http://www.sciencedirect.com/science/article/pii/S2405844023039270Streptococcus pyogenesGroup A StreptococcusHomologous recombinationGene deletionIsogenic mutant |
| spellingShingle | S. Boukthir P. Gaudu A. Faili S. Kayal pBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination Heliyon Streptococcus pyogenes Group A Streptococcus Homologous recombination Gene deletion Isogenic mutant |
| title | pBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination |
| title_full | pBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination |
| title_fullStr | pBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination |
| title_full_unstemmed | pBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination |
| title_short | pBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination |
| title_sort | pbfk a new thermosensitive shuttle vector for streptococcus pyogenes gene deletion by homologous recombination |
| topic | Streptococcus pyogenes Group A Streptococcus Homologous recombination Gene deletion Isogenic mutant |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023039270 |
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