pBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination
Streptococcus pyogenes, or Group A Streptococcus (GAS), is a major human pathogen for which genetic manipulation remains an ongoing challenge. We created a new temperature-sensitive plasmid pBFK expressing spectinomycin resistance adapted to homologous recombination procedure to perform a complete g...
Hlavní autoři: | , , , |
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Médium: | Článek |
Jazyk: | English |
Vydáno: |
Elsevier
2023-06-01
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Edice: | Heliyon |
Témata: | |
On-line přístup: | http://www.sciencedirect.com/science/article/pii/S2405844023039270 |