Simultaneous isolation of CD45 tumor-infiltrating lymphocytes, tumor cells, and associated fibroblasts from murine breast tumor model by MACS

Simultaneous isolation of CD45 tumor-infiltrating lymphocytes, tumor cells, and associated fibroblasts from murine breast tumor model by MACS

Summary: The study of the tumor microenvironment (TME) and its interactions with cancer cells is an important issue in cancer research. Here, we present a protocol to sort three important cell populations from murine triple negative breast cancer 4T1 model TME, including CD45+ tumor-infiltrating lym...

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Bibliographic Details
Main Authors: Laura Kalfeist, Stacy Petit, Loïck Galland, François Ghiringhelli, Sylvain Ladoire, Emeric Limagne
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:STAR Protocols
Subjects:
Cell isolation
Flow Cytometry/Mass Cytometry
Cancer
Immunology
Model Organisms
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722008310

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http://www.sciencedirect.com/science/article/pii/S2666166722008310

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