Generation and Characterization of CYP2E1-Overexpressing HepG2 Cells to Study the Role of CYP2E1 in Hepatic Hypoxia-Reoxygenation Injury

Generation and Characterization of CYP2E1-Overexpressing HepG2 Cells to Study the Role of CYP2E1 in Hepatic Hypoxia-Reoxygenation Injury

The mechanisms of hepatic ischemia/reperfusion (I/R) injury, which occurs during liver transplantation or surgery, are poorly understood. The purpose of the current study was to generate and characterize a HepG2 cell line with a stable overexpression of CYP2E1 to investigate the role of the enzyme i...

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Bibliographic Details
Main Authors: Nouf Alwadei, Mamunur Rashid, Devaraj Venkatapura Chandrashekar, Simin Rahighi, Jennifer Totonchy, Ajay Sharma, Reza Mehvar
Format: Article
Language:English
Published: MDPI AG 2023-05-01
Series:International Journal of Molecular Sciences
Subjects:
HepG2 cells
hepatic ischemia-reperfusion
hypoxia-reoxygenation
cytochrome P450
CYP2E1
reactive oxygen species
Online Access:https://www.mdpi.com/1422-0067/24/9/8121

Internet

https://www.mdpi.com/1422-0067/24/9/8121

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