Combined acid hydrolysis and fermentation improves bioactivity of citrus flavonoids in vitro and in vivo
Abstract Many bioactive plant compounds, known as phytochemicals, have the potential to improve health. Unfortunately, the bioavailability and bioactivity of phytochemicals such as polyphenolic flavonoids are reduced due to conjugation with sugar moieties. Here, we combine acid hydrolysis and tailor...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-10-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-023-05424-7 |
| _version_ | 1797557180385198080 |
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| author | Alice König Nadiia Sadova Marion Dornmayr Bettina Schwarzinger Cathrina Neuhauser Verena Stadlbauer Melanie Wallner Jakob Woischitzschläger Andreas Müller Rolf Tona Daniel Kofel Julian Weghuber |
| author_facet | Alice König Nadiia Sadova Marion Dornmayr Bettina Schwarzinger Cathrina Neuhauser Verena Stadlbauer Melanie Wallner Jakob Woischitzschläger Andreas Müller Rolf Tona Daniel Kofel Julian Weghuber |
| author_sort | Alice König |
| collection | DOAJ |
| description | Abstract Many bioactive plant compounds, known as phytochemicals, have the potential to improve health. Unfortunately, the bioavailability and bioactivity of phytochemicals such as polyphenolic flavonoids are reduced due to conjugation with sugar moieties. Here, we combine acid hydrolysis and tailored fermentation by lactic acid bacteria (Lactiplantibacillus plantarum) to convert the biologically less active flavonoid glycosides hesperidin and naringin into the more active aglycones hesperetin and naringenin. Using a comprehensive approach, we identify the most effective hydrolysis and fermentation conditions to increase the concentration of the aglycones in citrus extracts. The higher cellular transport and bioactivity of the biotransformed citrus extract are also demonstrated in vitro and in vivo. Superior antioxidant, anti-inflammatory and cell migration activities in vitro, as well as intestinal barrier protecting and antioxidant activities in Drosophila melanogaster are identified. In conclusion, the presented biotransformation approach improves the bioactivity of flavonoids, clearly traced back to the increase in aglycone content. |
| first_indexed | 2024-03-10T17:13:42Z |
| format | Article |
| id | doaj.art-f3eb255706da44dd9a866843596bf3b9 |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-03-10T17:13:42Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-f3eb255706da44dd9a866843596bf3b92023-11-20T10:36:14ZengNature PortfolioCommunications Biology2399-36422023-10-016111810.1038/s42003-023-05424-7Combined acid hydrolysis and fermentation improves bioactivity of citrus flavonoids in vitro and in vivoAlice König0Nadiia Sadova1Marion Dornmayr2Bettina Schwarzinger3Cathrina Neuhauser4Verena Stadlbauer5Melanie Wallner6Jakob Woischitzschläger7Andreas Müller8Rolf Tona9Daniel Kofel10Julian Weghuber11Center of Excellence Food Technology and Nutrition, University of Applied Sciences Upper AustriaCenter of Excellence Food Technology and Nutrition, University of Applied Sciences Upper AustriaCenter of Excellence Food Technology and Nutrition, University of Applied Sciences Upper AustriaCenter of Excellence Food Technology and Nutrition, University of Applied Sciences Upper AustriaCenter of Excellence Food Technology and Nutrition, University of Applied Sciences Upper AustriaCenter of Excellence Food Technology and Nutrition, University of Applied Sciences Upper AustriaCenter of Excellence Food Technology and Nutrition, University of Applied Sciences Upper AustriaCenter of Excellence Food Technology and Nutrition, University of Applied Sciences Upper AustriaTriPlant AGTriPlant AGTriPlant AGCenter of Excellence Food Technology and Nutrition, University of Applied Sciences Upper AustriaAbstract Many bioactive plant compounds, known as phytochemicals, have the potential to improve health. Unfortunately, the bioavailability and bioactivity of phytochemicals such as polyphenolic flavonoids are reduced due to conjugation with sugar moieties. Here, we combine acid hydrolysis and tailored fermentation by lactic acid bacteria (Lactiplantibacillus plantarum) to convert the biologically less active flavonoid glycosides hesperidin and naringin into the more active aglycones hesperetin and naringenin. Using a comprehensive approach, we identify the most effective hydrolysis and fermentation conditions to increase the concentration of the aglycones in citrus extracts. The higher cellular transport and bioactivity of the biotransformed citrus extract are also demonstrated in vitro and in vivo. Superior antioxidant, anti-inflammatory and cell migration activities in vitro, as well as intestinal barrier protecting and antioxidant activities in Drosophila melanogaster are identified. In conclusion, the presented biotransformation approach improves the bioactivity of flavonoids, clearly traced back to the increase in aglycone content.https://doi.org/10.1038/s42003-023-05424-7 |
| spellingShingle | Alice König Nadiia Sadova Marion Dornmayr Bettina Schwarzinger Cathrina Neuhauser Verena Stadlbauer Melanie Wallner Jakob Woischitzschläger Andreas Müller Rolf Tona Daniel Kofel Julian Weghuber Combined acid hydrolysis and fermentation improves bioactivity of citrus flavonoids in vitro and in vivo Communications Biology |
| title | Combined acid hydrolysis and fermentation improves bioactivity of citrus flavonoids in vitro and in vivo |
| title_full | Combined acid hydrolysis and fermentation improves bioactivity of citrus flavonoids in vitro and in vivo |
| title_fullStr | Combined acid hydrolysis and fermentation improves bioactivity of citrus flavonoids in vitro and in vivo |
| title_full_unstemmed | Combined acid hydrolysis and fermentation improves bioactivity of citrus flavonoids in vitro and in vivo |
| title_short | Combined acid hydrolysis and fermentation improves bioactivity of citrus flavonoids in vitro and in vivo |
| title_sort | combined acid hydrolysis and fermentation improves bioactivity of citrus flavonoids in vitro and in vivo |
| url | https://doi.org/10.1038/s42003-023-05424-7 |
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