Single-tube nested PCR assay with in-house DNA extraction for Mycobacterium tuberculosis detection in blood and urine

Single-tube nested PCR assay with in-house DNA extraction for Mycobacterium tuberculosis detection in blood and urine

Abstract: INTRODUCTION : Molecular analyses are auxiliary tools for detecting Koch's bacilli in clinical specimens from patients with suspected tuberculosis (TB). However, there are still no efficient diagnostic tests that combine high sensitivity and specificity and yield rapid results in the...

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Main Authors: Juliana Figueirêdo da Costa Lima, Gabriela de Moraes Rêgo Guedes, Juliana Falcão de Araújo Lima, Laís Ariane de Siqueira Lira, Fabiana Cristina Fulco Santos, Mercia Eliane de Arruda, Lílian Maria Lapa Montenegro, Haiana Charifker Schindler
Format: Article
Language:English
Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2015-12-01
Series:Revista da Sociedade Brasileira de Medicina Tropical
Subjects:
Mycobacterium tuberculosis
Molecular diagnostic test
Nested polymerase chain reaction
Blood
Urine
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000600731&lng=en&tlng=en

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000600731&lng=en&tlng=en

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