Cloning, Expression, and In Vitro Functional Activity Assay of phiC31 Integrase cDNA in Escherichia coli

Cloning, Expression, and In Vitro Functional Activity Assay of phiC31 Integrase cDNA in Escherichia coli

Objective: The aim of present study was cloning and expression of phiC31 integrase cDNA in a bacterial expression vector. Thus, an intra molecular assay vector was applied to show in vitro activity of recombinant protein.Materials and Methods: In this experimental study, phiC31 cDNA was subcloned in...

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Bibliographic Details
Main Authors: Mohammad Hadi Sekhavati, Mojtaba Tahmoorespur, Kamran Ghaedi, Kianoush Dormiani, Pharm, Mohammad Reza Nassiri, Yahya Khazaie, Mahboubeh Foruzanfar, Morteza Hosseini, Mohammad Hossein Nasr Esfahani
Format: Article
Language:English
Published: Royan Institute (ACECR), Tehran 2013-01-01
Series:Cell Journal
Subjects:
phiC31
Site-Specific Integration
E.coli BL21 (DE3)
Online Access:http://celljournal.org/library/upload/article/af_33756277423752622223626756247246557883623-Sekhavati-1.pdf

Internet

http://celljournal.org/library/upload/article/af_33756277423752622223626756247246557883623-Sekhavati-1.pdf

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