CryoFIB milling large tissue samples for cryo-electron tomography

Abstract Cryo-electron tomography (cryoET) is a powerful tool for exploring the molecular structure of large organisms. However, technical challenges still limit cryoET applications on large samples. In particular, localization and cutting out objects of interest from a large tissue sample are still...

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Bibliographic Details
Main Authors: Sihan Wang, Heng Zhou, Wei Chen, Yifeng Jiang, Xuzhen Yan, Hong You, Xueming Li
Format: Article
Language:English
Published: Nature Portfolio 2023-04-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-023-32716-z