A gene optimization strategy that enhances production of fully functional P-glycoprotein in Pichia pastoris.

A gene optimization strategy that enhances production of fully functional P-glycoprotein in Pichia pastoris.

Structural and biochemical studies of mammalian membrane proteins remain hampered by inefficient production of pure protein. We explored codon optimization based on highly expressed Pichia pastoris genes to enhance co-translational folding and production of P-glycoprotein (Pgp), an ATP-dependent dru...

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Bibliographic Details
Main Authors: Jiangping Bai, Douglas J Swartz, Irina I Protasevich, Christie G Brouillette, Patina M Harrell, Ellen Hildebrandt, Brigitte Gasser, Diethard Mattanovich, Andrew Ward, Geoffrey Chang, Ina L Urbatsch
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3149604?pdf=render

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http://europepmc.org/articles/PMC3149604?pdf=render

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