Protocol for the detection of defined T cell clones in a heterogeneous cell population

Summary: Identifying defined T cell clones within a polyclonal population is key to clarifying their phenotype and function. Here, we present a protocol for detecting specified T cell clones in a heterogeneous cell population. We describe steps for stimulating human CD4+ T cells isolated from blood...

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Bibliographic Details
Main Authors: Andrea Gobbini, Alessandra Bandera, Renata Grifantini, Sergio Abrignani, Samuele Notarbartolo
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723007542