Efficient Genome Editing in <i>Bacillus licheniformis</i> Mediated by a Conditional CRISPR/Cas9 System

<i>Bacillus licheniformis</i> is widely used to produce multiple enzymes and chemicals in industrial fermentation. It is also an organism that is hard to genetically manipulate, which is mainly attributed to its extremely low transformation efficiency. The lack of genetic modification te...

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Bibliographic Details
Main Authors: Youran Li, Hanrong Wang, Liang Zhang, Zhongyang Ding, Sha Xu, Zhenghua Gu, Guiyang Shi
Format: Article
Language:English
Published: MDPI AG 2020-05-01
Series:Microorganisms
Subjects:
Online Access:https://www.mdpi.com/2076-2607/8/5/754