Oxaliplatin treatment changes the function of sensory nerves in rats
Oxaliplatin (L-OHP) is a platinum-based chemotherapy drug, used in standard treatment of colorectal cancer. L-OHP frequently causes acute peripheral neuropathies. These adverse effects limit cancer therapy with L-OHP. The present study was designed to reveal the changes in sensory nerve function in...
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Elsevier
2016-04-01
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| Series: | Journal of Pharmacological Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861315002443 |
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| author | Shohei Yamamoto Hideki Ono Kazuhiko Kume Masahiro Ohsawa |
| author_facet | Shohei Yamamoto Hideki Ono Kazuhiko Kume Masahiro Ohsawa |
| author_sort | Shohei Yamamoto |
| collection | DOAJ |
| description | Oxaliplatin (L-OHP) is a platinum-based chemotherapy drug, used in standard treatment of colorectal cancer. L-OHP frequently causes acute peripheral neuropathies. These adverse effects limit cancer therapy with L-OHP. The present study was designed to reveal the changes in sensory nerve function in L-OHP-injected rats. Mechanical static allodynia, dynamic allodynia, and cold allodynia were evaluated using the von Frey test, brush test, and acetone test, respectively. Sensory nerve fiber responsiveness was measured using a Neurometer. The fifth lumbar ventral root was sectioned to record multi-unit efferent discharges. Single intraperitoneal administration of L-OHP induced mechanical static allodynia, dynamic allodynia, and cold allodynia in Wistar/ST rats. The thresholds for paw withdrawal induced by 2000 Hz (Aβ-fiber) and 5 Hz (C-fiber), but not 250 Hz (Aδ-fiber) sine-wave electrical stimulation were reduced in L-OHP-treated rats. Multi-unit efferent discharges were increased by mechanical stimulation using a von Frey filament applied to the plantar surface of the hindpaw. The discharges during and after stimulation were increased in the L-OHP-treated rats. Cold stimulation, but not brush stimulation, increased the discharges in L-OHP-treated rats. These results suggest that sensitization of Aβ- and C-fibers, but not Aδ-fibers, contributes to the development of L-OHP-induced mechanical and cold allodynia. |
| first_indexed | 2024-12-13T14:28:55Z |
| format | Article |
| id | doaj.art-f44cad3653584481a29bd94207a62c4b |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-13T14:28:55Z |
| publishDate | 2016-04-01 |
| publisher | Elsevier |
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| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-f44cad3653584481a29bd94207a62c4b2022-12-21T23:41:53ZengElsevierJournal of Pharmacological Sciences1347-86132016-04-01130418919310.1016/j.jphs.2015.12.004Oxaliplatin treatment changes the function of sensory nerves in ratsShohei Yamamoto0Hideki Ono1Kazuhiko Kume2Masahiro Ohsawa3Department of Neuropharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya 467-8603, JapanLaboratory of CNS Pharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, JapanDepartment of Neuropharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya 467-8603, JapanDepartment of Neuropharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya 467-8603, JapanOxaliplatin (L-OHP) is a platinum-based chemotherapy drug, used in standard treatment of colorectal cancer. L-OHP frequently causes acute peripheral neuropathies. These adverse effects limit cancer therapy with L-OHP. The present study was designed to reveal the changes in sensory nerve function in L-OHP-injected rats. Mechanical static allodynia, dynamic allodynia, and cold allodynia were evaluated using the von Frey test, brush test, and acetone test, respectively. Sensory nerve fiber responsiveness was measured using a Neurometer. The fifth lumbar ventral root was sectioned to record multi-unit efferent discharges. Single intraperitoneal administration of L-OHP induced mechanical static allodynia, dynamic allodynia, and cold allodynia in Wistar/ST rats. The thresholds for paw withdrawal induced by 2000 Hz (Aβ-fiber) and 5 Hz (C-fiber), but not 250 Hz (Aδ-fiber) sine-wave electrical stimulation were reduced in L-OHP-treated rats. Multi-unit efferent discharges were increased by mechanical stimulation using a von Frey filament applied to the plantar surface of the hindpaw. The discharges during and after stimulation were increased in the L-OHP-treated rats. Cold stimulation, but not brush stimulation, increased the discharges in L-OHP-treated rats. These results suggest that sensitization of Aβ- and C-fibers, but not Aδ-fibers, contributes to the development of L-OHP-induced mechanical and cold allodynia.http://www.sciencedirect.com/science/article/pii/S1347861315002443Oxaliplatin (L-OHP)Neuropathic painSensory nerveNeurometerEfferent discharge |
| spellingShingle | Shohei Yamamoto Hideki Ono Kazuhiko Kume Masahiro Ohsawa Oxaliplatin treatment changes the function of sensory nerves in rats Journal of Pharmacological Sciences Oxaliplatin (L-OHP) Neuropathic pain Sensory nerve Neurometer Efferent discharge |
| title | Oxaliplatin treatment changes the function of sensory nerves in rats |
| title_full | Oxaliplatin treatment changes the function of sensory nerves in rats |
| title_fullStr | Oxaliplatin treatment changes the function of sensory nerves in rats |
| title_full_unstemmed | Oxaliplatin treatment changes the function of sensory nerves in rats |
| title_short | Oxaliplatin treatment changes the function of sensory nerves in rats |
| title_sort | oxaliplatin treatment changes the function of sensory nerves in rats |
| topic | Oxaliplatin (L-OHP) Neuropathic pain Sensory nerve Neurometer Efferent discharge |
| url | http://www.sciencedirect.com/science/article/pii/S1347861315002443 |
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