Eukaryote-Specific Insertion Elements Control Human ARGONAUTE Slicer Activity

Eukaryote-Specific Insertion Elements Control Human ARGONAUTE Slicer Activity

We have solved the crystal structure of human ARGONAUTE1 (hAGO1) bound to endogenous 5′-phosphorylated guide RNAs. To identify changes that evolutionarily rendered hAGO1 inactive, we compared our structure with guide-RNA-containing and cleavage-active hAGO2. Aside from mutation of a catalytic tetrad...

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Bibliographic Details
Main Authors: Kotaro Nakanishi, Manuel Ascano, Tasos Gogakos, Satoko Ishibe-Murakami, Artem A. Serganov, Daniel Briskin, Pavel Morozov, Thomas Tuschl, Dinshaw J. Patel
Format: Article
Language:English
Published: Elsevier 2013-06-01
Series:Cell Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S221112471300288X

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http://www.sciencedirect.com/science/article/pii/S221112471300288X

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