Gas2l3, a novel constriction site-associated protein whose regulation is mediated by the APC/C Cdh1 complex.

Growth arrest-specific 2-like protein 3 (Gas2l3) was recently identified as an Actin/Tubulin cross-linker protein that regulates cytokinesis. Using cell-free systems from both frog eggs and human cells, we show that the Gas2l3 protein is targeted for ubiquitin-mediated proteolysis by the APC/C(Cdh1)...

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Main Authors: Tal Pe'er, Roxane Lahmi, Yaara Sharaby, Evelin Chorni, Meirav Noach, Manuela Vecsler, Eitan Zlotorynski, Hanno Steen, Judith A Steen, Amit Tzur
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3585356?pdf=render
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author Tal Pe'er
Roxane Lahmi
Yaara Sharaby
Evelin Chorni
Meirav Noach
Manuela Vecsler
Eitan Zlotorynski
Hanno Steen
Judith A Steen
Amit Tzur
author_facet Tal Pe'er
Roxane Lahmi
Yaara Sharaby
Evelin Chorni
Meirav Noach
Manuela Vecsler
Eitan Zlotorynski
Hanno Steen
Judith A Steen
Amit Tzur
author_sort Tal Pe'er
collection DOAJ
description Growth arrest-specific 2-like protein 3 (Gas2l3) was recently identified as an Actin/Tubulin cross-linker protein that regulates cytokinesis. Using cell-free systems from both frog eggs and human cells, we show that the Gas2l3 protein is targeted for ubiquitin-mediated proteolysis by the APC/C(Cdh1) complex, but not by the APC/C(Cdc20) complex, and is phosphorylated by Cdk1 in mitosis. Moreover, late in cytokinesis, Gas2l3 is exclusively localized to the constriction sites, which are the narrowest parts of the intercellular bridge connecting the two daughter cells. Overexpression of Gas2l3 specifically interferes with cell abscission, which is the final stage of cell division, when the cutting of the intercellular bridge at the constriction sites occurs. We therefore suggest that Gas2l3 is part of the cellular mechanism that terminates cell division.
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spelling doaj.art-f48e629369a843c99f86c6a4283d8c382022-12-22T01:14:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0182e5753210.1371/journal.pone.0057532Gas2l3, a novel constriction site-associated protein whose regulation is mediated by the APC/C Cdh1 complex.Tal Pe'erRoxane LahmiYaara SharabyEvelin ChorniMeirav NoachManuela VecslerEitan ZlotorynskiHanno SteenJudith A SteenAmit TzurGrowth arrest-specific 2-like protein 3 (Gas2l3) was recently identified as an Actin/Tubulin cross-linker protein that regulates cytokinesis. Using cell-free systems from both frog eggs and human cells, we show that the Gas2l3 protein is targeted for ubiquitin-mediated proteolysis by the APC/C(Cdh1) complex, but not by the APC/C(Cdc20) complex, and is phosphorylated by Cdk1 in mitosis. Moreover, late in cytokinesis, Gas2l3 is exclusively localized to the constriction sites, which are the narrowest parts of the intercellular bridge connecting the two daughter cells. Overexpression of Gas2l3 specifically interferes with cell abscission, which is the final stage of cell division, when the cutting of the intercellular bridge at the constriction sites occurs. We therefore suggest that Gas2l3 is part of the cellular mechanism that terminates cell division.http://europepmc.org/articles/PMC3585356?pdf=render
spellingShingle Tal Pe'er
Roxane Lahmi
Yaara Sharaby
Evelin Chorni
Meirav Noach
Manuela Vecsler
Eitan Zlotorynski
Hanno Steen
Judith A Steen
Amit Tzur
Gas2l3, a novel constriction site-associated protein whose regulation is mediated by the APC/C Cdh1 complex.
PLoS ONE
title Gas2l3, a novel constriction site-associated protein whose regulation is mediated by the APC/C Cdh1 complex.
title_full Gas2l3, a novel constriction site-associated protein whose regulation is mediated by the APC/C Cdh1 complex.
title_fullStr Gas2l3, a novel constriction site-associated protein whose regulation is mediated by the APC/C Cdh1 complex.
title_full_unstemmed Gas2l3, a novel constriction site-associated protein whose regulation is mediated by the APC/C Cdh1 complex.
title_short Gas2l3, a novel constriction site-associated protein whose regulation is mediated by the APC/C Cdh1 complex.
title_sort gas2l3 a novel constriction site associated protein whose regulation is mediated by the apc c cdh1 complex
url http://europepmc.org/articles/PMC3585356?pdf=render
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