Generation of a transgenic mouse embryonic stem cell line overexpressing DNMT1
DNMT1 overexpression is reported in disorders like schizophrenia, bipolar, epilepsy and multiple cancer types. Here, we used non-homologous recombination to generate R1Dnmt1WT-1, a mouse embryonic stem cell (ESC) line carrying a Dnmt1 cDNA transgene to achieve about two-fold overexpression. This ESC...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2023-09-01
|
Series: | Stem Cell Research |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506123001277 |