Generation of a transgenic mouse embryonic stem cell line overexpressing DNMT1

Generation of a transgenic mouse embryonic stem cell line overexpressing DNMT1

DNMT1 overexpression is reported in disorders like schizophrenia, bipolar, epilepsy and multiple cancer types. Here, we used non-homologous recombination to generate R1Dnmt1WT-1, a mouse embryonic stem cell (ESC) line carrying a Dnmt1 cDNA transgene to achieve about two-fold overexpression. This ESC...

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Bibliographic Details
Main Authors: Sumana Choudhury, Anuhya Anne, Purnima P. Pradhan, K. Naga Mohan
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:Stem Cell Research
Subjects:
DNMT1 overexpression
Schizophrenia
Cancers
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506123001277
Description
Summary:DNMT1 overexpression is reported in disorders like schizophrenia, bipolar, epilepsy and multiple cancer types. Here, we used non-homologous recombination to generate R1Dnmt1WT-1, a mouse embryonic stem cell (ESC) line carrying a Dnmt1 cDNA transgene to achieve about two-fold overexpression. This ESC line showed increased transcript levels of Sox2 pluripotency marker. R1Dnmt1WT-1 embryoid bodies showed increased levels of Lefty1 (endoderm), Tbxt and Acta2 (mesoderm), and Pax6 (ectoderm) transcripts. This new line showed normal karyotype and microsatellite profiles making it useful in studying carcinogenesis and abnormal neurogenesis due to DNMT1 overexpression.
ISSN:1873-5061

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