Generation of the transgene-free canker-resistant Citrus sinensis using Cas12a/crRNA ribonucleoprotein in the T0 generation

Abstract Citrus canker caused by Xanthomonas citri subsp. citri (Xcc) is a destructive citrus disease worldwide. Generating disease-resistant cultivars is the most effective, environmentally friendly and economic approach for disease control. However, citrus traditional breeding is lengthy and labor...

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Bibliographic Details
Main Authors: Hang Su, Yuanchun Wang, Jin Xu, Ahmad A. Omar, Jude W. Grosser, Milica Calovic, Liyang Zhang, Yu Feng, Christopher A. Vakulskas, Nian Wang
Format: Article
Language:English
Published: Nature Portfolio 2023-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-023-39714-9