Substrates incorporating unnatural amino acids are separately detectable for chymotrypsin and subtilisin with similar specificity

Protease specificity depends on the three-dimensional structure and sequence of the protease, and each protease plays a different role. Chymotrypsin and subtilisin have similar specificity for recognizing aromatic and hydrophobic amino acid residues. Previous methods using natural amino acids have d...

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Bibliographic Details
Main Authors: Yuuki Yamawaki, Tamaki Kato
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:Results in Chemistry
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2211715623004563