Modified protocol for culturing Drosophila S2 R+ cells and adult plasmatocytes to study actin cytoskeleton dynamics

Modified protocol for culturing Drosophila S2 R+ cells and adult plasmatocytes to study actin cytoskeleton dynamics

Summary: Here, we describe a protocol to culture Drosophila S2R+ cells and to extract plasmatocytes from adult flies. The modified seeding approach detailed here, in combination with coating of coverslips with concanvalin A, enables enhanced adhesion and spreading of cells. We describe the steps for...

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Bibliographic Details
Main Authors: Ceileigh M. Weaver, Stephanie Makdissi, Francesca Di Cara
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Cell Biology
Genetics
Immunology
Microscopy
Model Organisms
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722004683

Internet

http://www.sciencedirect.com/science/article/pii/S2666166722004683

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