Fluorometric Assay for DNA Polymerases and Reverse Transcriptase
We report a quick, easy and inexpensive fluorometric assay that measures the activity of replication enzymes using Pico-GreenTM. The systems tested include replication of the natural template M13 Gori by E. coli DNA polymerase III holoenzyme and the replication of a synthetic homopolymer by human im...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1996-10-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/96214st04 |