Studies on the high frequency plant regeneration from indica rice immature embryos and its transformation using biolistic method(籼稻幼胚组织培养高频率植株再生及其基因转化的研究)
以籼稻品种特青的幼胚作为外植体，研究了激素对愈伤组织诱导及植株再生的影响。MS+2,4-D 2 mg/L作培养基，愈伤组织诱导率达100%；愈伤组织在MS + KT 3 mg/L + NAA 0.5 mg/L的培养基上，分化频率为67.3%；在此基础上利用基因枪转化技术，将外源的bar基因导入了水稻基因组，转基因植株表现出对除草剂Basta很强的抗性。...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Zhejiang University Press
2000-07-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
| Subjects: | |
| Online Access: | https://doi.org/zjup/1008-9497.2000.27.4.469-472 |