Flow-Seq Evaluation of Translation Driven by a Set of Natural <i>Escherichia coli</i> 5′-UTR of Variable Length

Flow-Seq Evaluation of Translation Driven by a Set of Natural <i>Escherichia coli</i> 5′-UTR of Variable Length

Flow-seq is a method that combines fluorescently activated cell sorting and next-generation sequencing to deduce a large amount of data about translation efficiency from a single experiment. Here, we constructed a library of fluorescent protein-based reporters preceded by a set of 648 natural 5′-unt...

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Bibliographic Details
Main Authors: Ekaterina S. Komarova, Anna N. Slesarchuk, Maria P. Rubtsova, Ilya A. Osterman, Alexey E. Tupikin, Dmitry V. Pyshnyi, Olga A. Dontsova, Marsel R. Kabilov, Petr V. Sergiev
Format: Article
Language:English
Published: MDPI AG 2022-10-01
Series:International Journal of Molecular Sciences
Subjects:
ribosome
translation
Flow-seq
untranslated region
translation efficiency
Online Access:https://www.mdpi.com/1422-0067/23/20/12293

Internet

https://www.mdpi.com/1422-0067/23/20/12293

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