Evaluation of low-cost SARS-CoV-2 RNA purification methods for viral quantification by RT-qPCR and next-generation sequencing analysis: Implications for wider wastewater-based epidemiology adoption

Evaluation of low-cost SARS-CoV-2 RNA purification methods for viral quantification by RT-qPCR and next-generation sequencing analysis: Implications for wider wastewater-based epidemiology adoption

Wastewater: Based Epidemiology (WBE) consists of quantifying biomarkers in sewerage systems to derive real-time information on the health and/or lifestyle of the contributing population. WBE usefulness was vastly demonstrated in the context of the COVID-19 pandemic. Many methods for SARS-CoV-2 RNA d...

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Bibliographic Details
Main Authors: Alonso Reyes-Calderón, Elías Mindreau-Ganoza, Braulio Pardo-Figueroa, Katherine R. Garcia-Luquillas, Sonia P. Yufra, Pedro E. Romero, Claudia Antonini, Jose-Miguel Renom, Cesar R. Mota, Monica C. Santa-Maria
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:Heliyon
Subjects:
Wastewater-based epidemiology
SARS-CoV-2
COVID-19
Virus quantification
Viral genomic surveillance
RNA purification
Online Access:http://www.sciencedirect.com/science/article/pii/S2405844023033376

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http://www.sciencedirect.com/science/article/pii/S2405844023033376

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