Biocontrol of okra-rot-causing Cochliobolus spicifer-CSN-20 using secondary metabolites of endophytic fungi associated with Solenostemma arghel
Rot disease is responsible for serious economic losses related to okra (Abelmoschus esculentus) crops cultivated in Upper Egypt. Colonies with a consistent morphology were isolated from the infected okra stems and leaves and subjected to morphological and molecular examinations. The causal pathogen...
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Elsevier
2022-06-01
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| Series: | Annals of Agricultural Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0570178322000033 |
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| author | Fatma F. Abdel-Motaal Noha M. Kamel Magdi A. El-Sayed Mohamed Abou-Ellail |
| author_facet | Fatma F. Abdel-Motaal Noha M. Kamel Magdi A. El-Sayed Mohamed Abou-Ellail |
| author_sort | Fatma F. Abdel-Motaal |
| collection | DOAJ |
| description | Rot disease is responsible for serious economic losses related to okra (Abelmoschus esculentus) crops cultivated in Upper Egypt. Colonies with a consistent morphology were isolated from the infected okra stems and leaves and subjected to morphological and molecular examinations. The causal pathogen was identified as Cochliobolus spicifer based on morphological fungus descriptions, as well as on the amplified 28S rDNA and internal transcribed spacer region (ITS) sequences, which showed 99%–100% similarity to the sequences of C. spicifer-CSN-20 strains. The volatile and non-volatile organic compounds (VOCs and n-VOCs, respectively) produced by the endophytic fungi that are associated with the medicinal plant Solenostemma arghel, namely Fusarium solani-F4-1007, Penicillium verrucosum-F2-1006, and Aspergillus terreus-F5-1008, inhibited the growth of the C. spicifer pathogen by 34.2%,31.4%, and 30.5%, respectively. In total,27 VOCs were identified by GC/MS, among which eight were specific to A. terreus-F5-1008, eight to P. verrucosum-F2-1006, and three to F. solani-F4-1007, whereas nine VOCs were commonly produced by the three endophytic fungi. Moreover, F. solani-F4-1007-produced VOCs and n-VOCs exhibited the highest antifungal activity, with 37.27% and 37.1% inhibition against C. spicifer colony growth, respectively. The potent antifungal VOCs produced by F. solani-F4-1007 were identified as 3,4-dihydro-2 h-1,5-(3″-t-butyl) benzodioxepine, 4-(2-hydroxyethyl) phenol, and phenylethyl alcohol using GC/MS. Therefore, F. solani-F4-1007 was tested as a potential biocontrol agent against C. spicifer-CSN-20 using an in-planta assay. Okra plants treated with endophytic F. solani-F4-1007did not show any disease symptoms, whereas those that were not treated with F. solani-F4-1007 exhibited severe disease symptoms when challenged with inoculation of the C. spicifer pathogen. Our results demonstrated the contribution of the endophytic fungus F. solani-F4-1007 as a potential biocontrol agent against the C. spicifer pathogen, to improve okra growth. |
| first_indexed | 2024-04-13T13:41:54Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 0570-1783 |
| language | English |
| last_indexed | 2024-04-13T13:41:54Z |
| publishDate | 2022-06-01 |
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| spelling | doaj.art-f54bd94d208e4ecaa1f9b49ae88248412022-12-22T02:44:37ZengElsevierAnnals of Agricultural Sciences0570-17832022-06-016712433Biocontrol of okra-rot-causing Cochliobolus spicifer-CSN-20 using secondary metabolites of endophytic fungi associated with Solenostemma arghelFatma F. Abdel-Motaal0Noha M. Kamel1Magdi A. El-Sayed2Mohamed Abou-Ellail3Botany Department, Faculty of Science, Aswan University, Aswan 81528, Egypt; Unit of Environmental Studies and Development, Aswan University, Aswan 81528, Egypt; Corresponding author at: Botany Department, Faculty of Science, Aswan University, Aswan 81528, Egypt.Botany Department, Faculty of Science, Aswan University, Aswan 81528, Egypt; Unit of Environmental Studies and Development, Aswan University, Aswan 81528, EgyptBotany Department, Faculty of Science, Aswan University, Aswan 81528, Egypt; Molecular Biotechnology Program, Biological Sciences Department, Faculty of Science, Galala University, New Galala City, Suez, EgyptDepartment of Genetics, Faculty of Agriculture and Natural Resources, Aswan University, Aswan 81528, EgyptRot disease is responsible for serious economic losses related to okra (Abelmoschus esculentus) crops cultivated in Upper Egypt. Colonies with a consistent morphology were isolated from the infected okra stems and leaves and subjected to morphological and molecular examinations. The causal pathogen was identified as Cochliobolus spicifer based on morphological fungus descriptions, as well as on the amplified 28S rDNA and internal transcribed spacer region (ITS) sequences, which showed 99%–100% similarity to the sequences of C. spicifer-CSN-20 strains. The volatile and non-volatile organic compounds (VOCs and n-VOCs, respectively) produced by the endophytic fungi that are associated with the medicinal plant Solenostemma arghel, namely Fusarium solani-F4-1007, Penicillium verrucosum-F2-1006, and Aspergillus terreus-F5-1008, inhibited the growth of the C. spicifer pathogen by 34.2%,31.4%, and 30.5%, respectively. In total,27 VOCs were identified by GC/MS, among which eight were specific to A. terreus-F5-1008, eight to P. verrucosum-F2-1006, and three to F. solani-F4-1007, whereas nine VOCs were commonly produced by the three endophytic fungi. Moreover, F. solani-F4-1007-produced VOCs and n-VOCs exhibited the highest antifungal activity, with 37.27% and 37.1% inhibition against C. spicifer colony growth, respectively. The potent antifungal VOCs produced by F. solani-F4-1007 were identified as 3,4-dihydro-2 h-1,5-(3″-t-butyl) benzodioxepine, 4-(2-hydroxyethyl) phenol, and phenylethyl alcohol using GC/MS. Therefore, F. solani-F4-1007 was tested as a potential biocontrol agent against C. spicifer-CSN-20 using an in-planta assay. Okra plants treated with endophytic F. solani-F4-1007did not show any disease symptoms, whereas those that were not treated with F. solani-F4-1007 exhibited severe disease symptoms when challenged with inoculation of the C. spicifer pathogen. Our results demonstrated the contribution of the endophytic fungus F. solani-F4-1007 as a potential biocontrol agent against the C. spicifer pathogen, to improve okra growth.http://www.sciencedirect.com/science/article/pii/S0570178322000033Abelmoschus esculentusCochliobolus spiciferEndophyteRot diseaseSolenostemma arghelVolatiles |
| spellingShingle | Fatma F. Abdel-Motaal Noha M. Kamel Magdi A. El-Sayed Mohamed Abou-Ellail Biocontrol of okra-rot-causing Cochliobolus spicifer-CSN-20 using secondary metabolites of endophytic fungi associated with Solenostemma arghel Annals of Agricultural Sciences Abelmoschus esculentus Cochliobolus spicifer Endophyte Rot disease Solenostemma arghel Volatiles |
| title | Biocontrol of okra-rot-causing Cochliobolus spicifer-CSN-20 using secondary metabolites of endophytic fungi associated with Solenostemma arghel |
| title_full | Biocontrol of okra-rot-causing Cochliobolus spicifer-CSN-20 using secondary metabolites of endophytic fungi associated with Solenostemma arghel |
| title_fullStr | Biocontrol of okra-rot-causing Cochliobolus spicifer-CSN-20 using secondary metabolites of endophytic fungi associated with Solenostemma arghel |
| title_full_unstemmed | Biocontrol of okra-rot-causing Cochliobolus spicifer-CSN-20 using secondary metabolites of endophytic fungi associated with Solenostemma arghel |
| title_short | Biocontrol of okra-rot-causing Cochliobolus spicifer-CSN-20 using secondary metabolites of endophytic fungi associated with Solenostemma arghel |
| title_sort | biocontrol of okra rot causing cochliobolus spicifer csn 20 using secondary metabolites of endophytic fungi associated with solenostemma arghel |
| topic | Abelmoschus esculentus Cochliobolus spicifer Endophyte Rot disease Solenostemma arghel Volatiles |
| url | http://www.sciencedirect.com/science/article/pii/S0570178322000033 |
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