Efficient Purification of Polyhistidine-Tagged Recombinant Proteins Using Functionalized Corundum Particles

Efficient Purification of Polyhistidine-Tagged Recombinant Proteins Using Functionalized Corundum Particles

Immobilized metal affinity chromatography (IMAC) is a popular and valuable method for the affinity purification of polyhistidine-tagged recombinant proteins. However, it often shows practical limitations, which might require cumbersome optimizations, additional polishing, and enrichment steps. Here,...

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Bibliographic Details
Main Authors: Jule L. Völzke, Sarah Smatty, Sarah Döring, Shireen Ewald, Marcus Oelze, Franziska Fratzke, Sabine Flemig, Zoltán Konthur, Michael G. Weller
Format: Article
Language:English
Published: MDPI AG 2023-05-01
Series:BioTech
Subjects:
aluminum oxide
sapphire
ethylenediaminetetraacetic acid
nickel chelate
EDTAD
hexaHis-Tag
Online Access:https://www.mdpi.com/2673-6284/12/2/31

Internet

https://www.mdpi.com/2673-6284/12/2/31

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