A recombinant 9E1 monoclonal antibody binds membrane and soluble channel catfish immunoglobulin M

The development and validation of the recombinant 9E1 monoclonal antibody against channel catfish IgM is described. The variable heavy and light chain domains of the 9E1 hybridoma were cloned into murine IgG1 and IgK expression vectors. These expression plasmids were co-transfected into 293F cells a...

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Bibliographic Details
Main Authors: Miles D. Lange, Emily M. Churchman, Allison L. Wise, Timothy J. Bruce
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:Fish and Shellfish Immunology Reports
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2667011923000063