Development of an efficient, non-viral transfection method for studying gene function and bone growth in human primary cranial suture mesenchymal cells reveals that the cells respond to BMP2 and BMP3

Development of an efficient, non-viral transfection method for studying gene function and bone growth in human primary cranial suture mesenchymal cells reveals that the cells respond to BMP2 and BMP3

<p>Abstract</p> <p>Background</p> <p>Achieving efficient introduction of plasmid DNA into primary cultures of mammalian cells is a common problem in biomedical research. Human primary cranial suture cells are derived from the connective mesenchymal tissue between the bo...

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Bibliographic Details
Main Authors: Dwivedi Prem P, Anderson Peter J, Powell Barry C
Format: Article
Language:English
Published: BMC 2012-08-01
Series:BMC Biotechnology
Subjects:
Transfection
Nucleofection
Skull
Bone
Primary cell culture
Mesenchymal
BMP2
luciferase
Online Access:http://www.biomedcentral.com/1472-6750/12/45

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http://www.biomedcentral.com/1472-6750/12/45

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