A White Plaque, Associated with Genomic Deletion, Derived from M13KE-Based Peptide Library Is Enriched in a Target-Unrelated Manner during Phage Display Biopanning Due to Propagation Advantage

A White Plaque, Associated with Genomic Deletion, Derived from M13KE-Based Peptide Library Is Enriched in a Target-Unrelated Manner during Phage Display Biopanning Due to Propagation Advantage

The nonspecific enrichment of target-unrelated peptides during biopanning remains a major drawback for phage display technology. The commercial Ph.D.<sup>TM</sup>-7 phage display library is used extensively for peptide discovery. This library is based on the M13KE vector, which carries t...

Full description

Bibliographic Details
Main Authors: Danna Kamstrup Sell, Ane Beth Sloth, Babak Bakhshinejad, Andreas Kjaer
Format: Article
Language:English
Published: MDPI AG 2022-03-01
Series:International Journal of Molecular Sciences
Subjects:
biopanning
competitive propagation
genomic deletion
lacZα sequence
M13KE
phage display
Online Access:https://www.mdpi.com/1422-0067/23/6/3308

Internet

https://www.mdpi.com/1422-0067/23/6/3308

Similar Items