Dysregulated Expression of CD28 and CTLA-4 Molecules in Patients with Acute Myeloid Leukemia and Possible Association with Development of Graft <em>versus</em> Host Disease after Hematopoietic Stem Cell Transplantation
Background: Dysregulated expression of co-stimulatory molecules is one of the immune escape mechanisms employed in hematologic malignancies like acute myeloid leukemia (AML). Objective: To evaluate the expression of the CD28 and CTLA-4 molecules in 62 adults with de novo AML and its correlation wit...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2019-05-01
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| Series: | International Journal of Organ Transplantation Medicine |
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| Online Access: | http://www.ijotm.com/ojs/index.php/IJOTM/article/view/610 |
| _version_ | 1811334416181493760 |
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| author | M Ramzi M Iravani Saadi R Yaghobi N Arandi |
| author_facet | M Ramzi M Iravani Saadi R Yaghobi N Arandi |
| author_sort | M Ramzi |
| collection | DOAJ |
| description | Background: Dysregulated expression of co-stimulatory molecules is one of the immune escape mechanisms employed in hematologic malignancies like acute myeloid leukemia (AML).
Objective: To evaluate the expression of the CD28 and CTLA-4 molecules in 62 adults with de novo AML and its correlation with the development of acute graft vs host disease (GVHD) after hematopoietic stem-cell transplantation.
Methods: The relative expression of CD28 and CTLA-4 was measured by quantitative SYBR Green real-time PCR method in a group of patients and controls as well as different risk groups (high, intermediate and favorite risk), M3 vs non-M3 and GVHD vs non-GVHD patients.
Results: The mRNA expression of CD28 (7.9-fold) and CTLA-4 (5.7-fold) was significantly increased in AML patients compared with healthy controls (p=0.006 and 0.02, respectively). Although the mean expression of both CD28 and CTLA-4 was increased in high-risk group compared with low-risk and intermediate- risk groups, the difference was not statistically significant. Also, the mean expression of the CTLA-4, but not CD28, was significantly higher in M3 patients compared with non-M3 ones (p<0.001). The expression of CD28 was upregulated in GVHD patients, while the expression of CTLA-4 was slightly lower in GVHD patients compared with non-GVHD patients, though the difference was not statistically significant. There was no significant correlation between the expression of CD28 and CTLA-4 and laboratory parameters like white blood cells and platelets counts, and hemoglobin and lactate dehydrogenase level in AML patients.
Conclusion: CD28 and CTLA-4 molecules are aberrantly expressed in peripheral blood leukocytes of AML patients and might contribute to the development of aGVHD after hematopoietic stem cell transplantation. |
| first_indexed | 2024-04-13T17:07:38Z |
| format | Article |
| id | doaj.art-f581f86449dd480fb6ad1df502d3aac7 |
| institution | Directory Open Access Journal |
| issn | 2008-6482 2008-6490 |
| language | English |
| last_indexed | 2024-04-13T17:07:38Z |
| publishDate | 2019-05-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | International Journal of Organ Transplantation Medicine |
| spelling | doaj.art-f581f86449dd480fb6ad1df502d3aac72022-12-22T02:38:25ZengShiraz University of Medical SciencesInternational Journal of Organ Transplantation Medicine2008-64822008-64902019-05-01102299Dysregulated Expression of CD28 and CTLA-4 Molecules in Patients with Acute Myeloid Leukemia and Possible Association with Development of Graft <em>versus</em> Host Disease after Hematopoietic Stem Cell TransplantationM Ramzi0M Iravani Saadi1R Yaghobi2N Arandi3Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, IranHematology Research Center, Shiraz University of Medical Sciences, Shiraz, IranShiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, IranHematology Research Center, Shiraz University of Medical Sciences, Shiraz, IranBackground: Dysregulated expression of co-stimulatory molecules is one of the immune escape mechanisms employed in hematologic malignancies like acute myeloid leukemia (AML). Objective: To evaluate the expression of the CD28 and CTLA-4 molecules in 62 adults with de novo AML and its correlation with the development of acute graft vs host disease (GVHD) after hematopoietic stem-cell transplantation. Methods: The relative expression of CD28 and CTLA-4 was measured by quantitative SYBR Green real-time PCR method in a group of patients and controls as well as different risk groups (high, intermediate and favorite risk), M3 vs non-M3 and GVHD vs non-GVHD patients. Results: The mRNA expression of CD28 (7.9-fold) and CTLA-4 (5.7-fold) was significantly increased in AML patients compared with healthy controls (p=0.006 and 0.02, respectively). Although the mean expression of both CD28 and CTLA-4 was increased in high-risk group compared with low-risk and intermediate- risk groups, the difference was not statistically significant. Also, the mean expression of the CTLA-4, but not CD28, was significantly higher in M3 patients compared with non-M3 ones (p<0.001). The expression of CD28 was upregulated in GVHD patients, while the expression of CTLA-4 was slightly lower in GVHD patients compared with non-GVHD patients, though the difference was not statistically significant. There was no significant correlation between the expression of CD28 and CTLA-4 and laboratory parameters like white blood cells and platelets counts, and hemoglobin and lactate dehydrogenase level in AML patients. Conclusion: CD28 and CTLA-4 molecules are aberrantly expressed in peripheral blood leukocytes of AML patients and might contribute to the development of aGVHD after hematopoietic stem cell transplantation.http://www.ijotm.com/ojs/index.php/IJOTM/article/view/610Acute graft versus host disease (aGVHD)AMLCo-stimulatory moleculesHematopoietic stem cell transplantation (HSCT) |
| spellingShingle | M Ramzi M Iravani Saadi R Yaghobi N Arandi Dysregulated Expression of CD28 and CTLA-4 Molecules in Patients with Acute Myeloid Leukemia and Possible Association with Development of Graft <em>versus</em> Host Disease after Hematopoietic Stem Cell Transplantation International Journal of Organ Transplantation Medicine Acute graft versus host disease (aGVHD) AML Co-stimulatory molecules Hematopoietic stem cell transplantation (HSCT) |
| title | Dysregulated Expression of CD28 and CTLA-4 Molecules in Patients with Acute Myeloid Leukemia and Possible Association with Development of Graft <em>versus</em> Host Disease after Hematopoietic Stem Cell Transplantation |
| title_full | Dysregulated Expression of CD28 and CTLA-4 Molecules in Patients with Acute Myeloid Leukemia and Possible Association with Development of Graft <em>versus</em> Host Disease after Hematopoietic Stem Cell Transplantation |
| title_fullStr | Dysregulated Expression of CD28 and CTLA-4 Molecules in Patients with Acute Myeloid Leukemia and Possible Association with Development of Graft <em>versus</em> Host Disease after Hematopoietic Stem Cell Transplantation |
| title_full_unstemmed | Dysregulated Expression of CD28 and CTLA-4 Molecules in Patients with Acute Myeloid Leukemia and Possible Association with Development of Graft <em>versus</em> Host Disease after Hematopoietic Stem Cell Transplantation |
| title_short | Dysregulated Expression of CD28 and CTLA-4 Molecules in Patients with Acute Myeloid Leukemia and Possible Association with Development of Graft <em>versus</em> Host Disease after Hematopoietic Stem Cell Transplantation |
| title_sort | dysregulated expression of cd28 and ctla 4 molecules in patients with acute myeloid leukemia and possible association with development of graft em versus em host disease after hematopoietic stem cell transplantation |
| topic | Acute graft versus host disease (aGVHD) AML Co-stimulatory molecules Hematopoietic stem cell transplantation (HSCT) |
| url | http://www.ijotm.com/ojs/index.php/IJOTM/article/view/610 |
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