Protocol for RNA fluorescence in situ hybridization in mouse meningeal whole mounts

Protocol for RNA fluorescence in situ hybridization in mouse meningeal whole mounts

Summary: The multilayered meninges surrounding the brain and spinal cord harbor distinct immune cell populations with prominent roles in health and diseases. Here we present an optimized protocol for RNA fluorescence in situ hybridization (RNA FISH) in meningeal whole mounts, allowing the visualizat...

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Bibliographic Details
Main Authors: Olof Rickard Nilsson, Laszlo Kari, Rebecca Rosenke, Olivia Steele-Mortimer
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Immunology
Microscopy
Antibody
In Situ Hybridization
Neuroscience
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722001368

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http://www.sciencedirect.com/science/article/pii/S2666166722001368

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