Preparing Arabidopsis thaliana root protoplasts for cryo electron tomography

The use of protoplasts in plant biology has become a convenient tool for the application of transient gene expression. This model system has allowed the study of plant responses to biotic and abiotic stresses, protein location and trafficking, cell wall dynamics, and single-cell transcriptomics, amo...

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Bibliographic Details
Main Authors: Ingrid Berenice Sanchez Carrillo, Patrick C. Hoffmann, Teura Barff, Martin Beck, Hugo Germain
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-09-01
Series:Frontiers in Plant Science
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2023.1261180/full