Recombinant expression of an l‐amino acid oxidase from the fungus Hebeloma cylindrosporum in Pichia pastoris including fermentation
Abstract l‐amino acid oxidases (LAAOs) are flavoenzymes that catalyze the oxidative deamination of l‐amino acids to the corresponding α‐keto acids, ammonia, and hydrogen peroxide. Here, we show the overexpression, purification, and the characterization of LAAO4 from the fungus Hebeloma cylindrosporu...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-10-01
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| Series: | MicrobiologyOpen |
| Online Access: | https://doi.org/10.1002/mbo3.1112 |