Chaperone assisted recombinant expression of a mycobacterial aminoacylase in Vibrio natriegens and Escherichia coli capable of N-lauroyl-L-amino acid synthesis

Chaperone assisted recombinant expression of a mycobacterial aminoacylase in Vibrio natriegens and Escherichia coli capable of N-lauroyl-L-amino acid synthesis

Abstract Background Aminoacylases are highly promising enzymes for the green synthesis of acyl-amino acids, potentially replacing the environmentally harmful Schotten-Baumann reaction. Long-chain acyl-amino acids can serve as strong surfactants and emulsifiers, with application in cosmetic industrie...

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Bibliographic Details
Main Authors: Gerrit Haeger, Jessika Wirges, Nicole Tanzmann, Sven Oyen, Tristan Jolmes, Karl-Erich Jaeger, Ulrich Schörken, Johannes Bongaerts, Petra Siegert
Format: Article
Language:English
Published: BMC 2023-04-01
Series:Microbial Cell Factories
Subjects:
Aminoacylase
Vibrio natriegens
Chaperone co-expression
Inclusion bodies
Acyl-amino acids
Online Access:https://doi.org/10.1186/s12934-023-02079-1

Internet

https://doi.org/10.1186/s12934-023-02079-1

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