Multi-factorial examination of amplicon sequencing workflows from sample preparation to bioinformatic analysis

Abstract Background The development of sequencing technologies to evaluate bacterial microbiota composition has allowed new insights into the importance of microbial ecology. However, the variety of methodologies used among amplicon sequencing workflows leads to uncertainty about best practices as w...

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Bibliographic Details
Main Authors: Travis J. De Wolfe, Erik S. Wright
Format: Article
Language:English
Published: BMC 2023-04-01
Series:BMC Microbiology
Subjects:
Online Access:https://doi.org/10.1186/s12866-023-02851-8