水酶法提取紫海胆性腺脂质的工艺优化 及提取方法对其品质的影响Process optimization of lipid extraction from the gonad of Heliocidaris crassispina via enzyme-assisted aqueous method and effect of extraction methods on its quality

以福建紫海胆(Heliocidaris crassispina)性腺为研究对象,采用单因素实验和正交实验优化水酶法提取紫海胆性腺脂质工艺,并基于气相色谱-质谱联用(GC-MS)、二醇基硅胶固相萃取(Generik Diol SPE)、高效薄层色谱(HPTLC)和差示扫描量热(DSC)等技术,以脂质得率、理化性质、脂肪酸组成、营养指标、脂质组成、熔融与结晶特性为指标,探讨了水酶法、Folch法和Matyash法对紫海胆性腺脂质品质的影响。结果表明:水酶法提取紫海胆性腺脂质最佳工艺条件为料液比1∶ 20、中性蛋白酶添加量7 500 U/g、酶解时间5 h、酶解温度50 ℃和pH 7,该条件下脂质得...

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Bibliographic Details
Main Author: 潘南1,江婷婷2,陈晓婷1,徐清云3,吴靖娜4,5,蔡水淋1,6,廖登远1,刘智禹1 PAN Nan1, JIANG Tingting2, CHEN Xiaoting1, XU Qingyun3, WU Jingna4,5, CAI Shuilin1,6, LIAO Dengyuan1, LIU Zhiyu1
Format: Article
Language:English
Published: 中粮工科(西安)国际工程有限公司 2022-03-01
Series:Zhongguo youzhi
Subjects:
Online Access:http://tg.chinaoils.cn/ch/reader/create_pdf.aspx?file_no=20220304&year_id=2022&quarter_id=3&falg=1