水酶法提取紫海胆性腺脂质的工艺优化 及提取方法对其品质的影响Process optimization of lipid extraction from the gonad of Heliocidaris crassispina via enzyme-assisted aqueous method and effect of extraction methods on its quality
以福建紫海胆（Heliocidaris crassispina）性腺为研究对象，采用单因素实验和正交实验优化水酶法提取紫海胆性腺脂质工艺，并基于气相色谱-质谱联用（GC-MS）、二醇基硅胶固相萃取（Generik Diol SPE）、高效薄层色谱（HPTLC）和差示扫描量热（DSC）等技术，以脂质得率、理化性质、脂肪酸组成、营养指标、脂质组成、熔融与结晶特性为指标，探讨了水酶法、Folch法和Matyash法对紫海胆性腺脂质品质的影响。结果表明：水酶法提取紫海胆性腺脂质最佳工艺条件为料液比1∶ 20、中性蛋白酶添加量7 500 U/g、酶解时间5 h、酶解温度50 ℃和pH 7，该条件下脂质得...
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中粮工科（西安）国际工程有限公司
2022-03-01
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| Series: | Zhongguo youzhi |
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| Online Access: | http://tg.chinaoils.cn/ch/reader/create_pdf.aspx?file_no=20220304&year_id=2022&quarter_id=3&falg=1 |
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| author | 潘南1，江婷婷2，陈晓婷1，徐清云3，吴靖娜4,5，蔡水淋1,6，廖登远1，刘智禹1 PAN Nan1， JIANG Tingting2， CHEN Xiaoting1， XU Qingyun3， WU Jingna4,5， CAI Shuilin1,6， LIAO Dengyuan1， LIU Zhiyu1 |
| author_facet | 潘南1，江婷婷2，陈晓婷1，徐清云3，吴靖娜4,5，蔡水淋1,6，廖登远1，刘智禹1 PAN Nan1， JIANG Tingting2， CHEN Xiaoting1， XU Qingyun3， WU Jingna4,5， CAI Shuilin1,6， LIAO Dengyuan1， LIU Zhiyu1 |
| author_sort | 潘南1，江婷婷2，陈晓婷1，徐清云3，吴靖娜4,5，蔡水淋1,6，廖登远1，刘智禹1 PAN Nan1， JIANG Tingting2， CHEN Xiaoting1， XU Qingyun3， WU Jingna4,5， CAI Shuilin1,6， LIAO Dengyuan1， LIU Zhiyu1 |
| collection | DOAJ |
| description | 以福建紫海胆（Heliocidaris crassispina）性腺为研究对象，采用单因素实验和正交实验优化水酶法提取紫海胆性腺脂质工艺，并基于气相色谱-质谱联用（GC-MS）、二醇基硅胶固相萃取（Generik Diol SPE）、高效薄层色谱（HPTLC）和差示扫描量热（DSC）等技术，以脂质得率、理化性质、脂肪酸组成、营养指标、脂质组成、熔融与结晶特性为指标，探讨了水酶法、Folch法和Matyash法对紫海胆性腺脂质品质的影响。结果表明：水酶法提取紫海胆性腺脂质最佳工艺条件为料液比1∶ 20、中性蛋白酶添加量7 500 U/g、酶解时间5 h、酶解温度50 ℃和pH 7，该条件下脂质得率为2322%，显著低于Folch法（27.27%）和Matyash法（27.14%）；与Folch法和Matyash法相比，水酶法提取的紫海胆性腺脂质过氧化值（0.006 g/100 g）较低，碘值（95.77 g/100 g）较高，饱和脂肪酸含量（51.48%）较低，多不饱和脂肪酸含量（34.53%）较高，EPA与DHA总含量（15.04%）较高，营养指标较好，磷脂含量（4.23%）较低，熔融与结晶温度较低。综上，虽然水酶法提取紫海胆性腺脂质工艺存在得率低、破乳工艺复杂、磷脂选择性差等技术瓶颈，但其反应条件温和、绿色安全，所提取的脂质品质佳，具有较好的营养价值、较低的熔融与结晶温度，适合高品质海洋膳食脂质的研制。
The lipids from the gonad of Heliocidaris crassispina (Fujian Province, China) was extracted using enzyme-assisted aqueous extraction method (EAE), and the extraction process was optimized by single factor experiment and orthogonal experiment. By applying gas chromatography-mass spectrometry (GC-MS), Generik Diol solid-phase extraction (SPE), high-performance thin layer chromatography (HPTLC) and differential scanning calorimetry (DSC) technologies, the quality of EAE lipid was comparatively characterized with that of Folch method and Matyash method with the lipid yields, physicochemical properties, fatty acid compositions, nutritional indexes, lipid compositions, melting and crystallization characteristics as indicators. The results showed that the optimal conditions of EAE process were determined as follows: solid-liquid ratio 1∶ 20, neutral protease dosage 7 500 U/g, enzymatic hydrolysis temperature 50 ℃，enzymatic hydrolysis time 5 h, and pH 7. The lipid yield was 23.22% under above conditions, which was significantly lower than that of Folch method (2727%) and Matyash method (27.14%). Compared with Folch method and Matyash method, the lipids from the gonad of Heliocidaris crassispina extracted by EAE had lower peroxide value (0.006 g/100 g), higher iodine value (95.77 gI/100 g), lower content of saturated fatty acids (51.48%), higher content of polyunsaturated fatty acids (34.53%), higher total content of EPA and DHA (15.04%), better nutritional indexes, lower phospholipids content (4.23%) and lower melting temperature and crystallization temperature. In conclusion, though bearing the shortages of low yield, complicated demulsification process and poor selectivity of phospholipids, EAE surpassed Folch method and Matyash method for milder conditions, eco-friendly initiatives, better quality, better nutritional value, lower melting and crystallization temperature of lipids, and suitable for the development of high quality marine dietary lipids. |
| first_indexed | 2024-03-12T17:05:36Z |
| format | Article |
| id | doaj.art-f66433def58b4117b9723cc8d58dd773 |
| institution | Directory Open Access Journal |
| issn | 1003-7969 |
| language | English |
| last_indexed | 2024-03-12T17:05:36Z |
| publishDate | 2022-03-01 |
| publisher | 中粮工科（西安）国际工程有限公司 |
| record_format | Article |
| series | Zhongguo youzhi |
| spelling | doaj.art-f66433def58b4117b9723cc8d58dd7732023-08-07T02:06:12Zeng中粮工科（西安）国际工程有限公司Zhongguo youzhi1003-79692022-03-014731826，4610.19902/j.cnki.zgyz.1003-7969.210120水酶法提取紫海胆性腺脂质的工艺优化 及提取方法对其品质的影响Process optimization of lipid extraction from the gonad of Heliocidaris crassispina via enzyme-assisted aqueous method and effect of extraction methods on its quality潘南1，江婷婷2，陈晓婷1，徐清云3，吴靖娜4,5，蔡水淋1,6，廖登远1，刘智禹1 PAN Nan1， JIANG Tingting2， CHEN Xiaoting1， XU Qingyun3， WU Jingna4,5， CAI Shuilin1,6， LIAO Dengyuan1， LIU Zhiyu101.福建省水产研究所 国家海水鱼类加工技术研发分中心，福建省海洋生物增养殖与高值化 利用重点实验室，福建 厦门 361013； 2.闽南师范大学 生物科学与技术学院，福建 漳州 363000； 3.厦门软件职业技术学院 软件工程系，福建 厦门 361024； 4.厦门医学院 厦门市海洋药用天然 产物资源重点实验室，福建 厦门 361023； 5.厦门医学院 海洋生物医药资源福建省高校工程 研究中心，福建 厦门 361023； 6.华侨大学 化工学院，福建 厦门 3610211.Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, National Research and Development Center for Marine Fish Processing, Fisheries Research Institute of Fujian, Xiamen 361013, Fujian, China; 2.School of Biological Science and Biotechnology, Minnan Normal University, Zhangzhou 363000, Fujian,China; 3.Department of Software Engineering, Xiamen Institute of Software Technology, Xiamen 361024, Fujian,China; 4.Xiamen Key Laboratory of Marine Medicinal Natural Products Resources, Xiamen Medical College, Xiamen 361023, Fujian,China; 5.Fujian Universities and Colleges Engineering Research Center of Marine Biophamaceutical Resources, Xiamen Medical College, Xiamen 361023, Fujian, China; 6.College of Chemical Engineering, Huaqiao University, Xiamen 361021, Fujian,China以福建紫海胆（Heliocidaris crassispina）性腺为研究对象，采用单因素实验和正交实验优化水酶法提取紫海胆性腺脂质工艺，并基于气相色谱-质谱联用（GC-MS）、二醇基硅胶固相萃取（Generik Diol SPE）、高效薄层色谱（HPTLC）和差示扫描量热（DSC）等技术，以脂质得率、理化性质、脂肪酸组成、营养指标、脂质组成、熔融与结晶特性为指标，探讨了水酶法、Folch法和Matyash法对紫海胆性腺脂质品质的影响。结果表明：水酶法提取紫海胆性腺脂质最佳工艺条件为料液比1∶ 20、中性蛋白酶添加量7 500 U/g、酶解时间5 h、酶解温度50 ℃和pH 7，该条件下脂质得率为2322%，显著低于Folch法（27.27%）和Matyash法（27.14%）；与Folch法和Matyash法相比，水酶法提取的紫海胆性腺脂质过氧化值（0.006 g/100 g）较低，碘值（95.77 g/100 g）较高，饱和脂肪酸含量（51.48%）较低，多不饱和脂肪酸含量（34.53%）较高，EPA与DHA总含量（15.04%）较高，营养指标较好，磷脂含量（4.23%）较低，熔融与结晶温度较低。综上，虽然水酶法提取紫海胆性腺脂质工艺存在得率低、破乳工艺复杂、磷脂选择性差等技术瓶颈，但其反应条件温和、绿色安全，所提取的脂质品质佳，具有较好的营养价值、较低的熔融与结晶温度，适合高品质海洋膳食脂质的研制。 The lipids from the gonad of Heliocidaris crassispina (Fujian Province, China) was extracted using enzyme-assisted aqueous extraction method (EAE), and the extraction process was optimized by single factor experiment and orthogonal experiment. By applying gas chromatography-mass spectrometry (GC-MS), Generik Diol solid-phase extraction (SPE), high-performance thin layer chromatography (HPTLC) and differential scanning calorimetry (DSC) technologies, the quality of EAE lipid was comparatively characterized with that of Folch method and Matyash method with the lipid yields, physicochemical properties, fatty acid compositions, nutritional indexes, lipid compositions, melting and crystallization characteristics as indicators. The results showed that the optimal conditions of EAE process were determined as follows: solid-liquid ratio 1∶ 20, neutral protease dosage 7 500 U/g, enzymatic hydrolysis temperature 50 ℃，enzymatic hydrolysis time 5 h, and pH 7. The lipid yield was 23.22% under above conditions, which was significantly lower than that of Folch method (2727%) and Matyash method (27.14%). Compared with Folch method and Matyash method, the lipids from the gonad of Heliocidaris crassispina extracted by EAE had lower peroxide value (0.006 g/100 g), higher iodine value (95.77 gI/100 g), lower content of saturated fatty acids (51.48%), higher content of polyunsaturated fatty acids (34.53%), higher total content of EPA and DHA (15.04%), better nutritional indexes, lower phospholipids content (4.23%) and lower melting temperature and crystallization temperature. In conclusion, though bearing the shortages of low yield, complicated demulsification process and poor selectivity of phospholipids, EAE surpassed Folch method and Matyash method for milder conditions, eco-friendly initiatives, better quality, better nutritional value, lower melting and crystallization temperature of lipids, and suitable for the development of high quality marine dietary lipids.http://tg.chinaoils.cn/ch/reader/create_pdf.aspx?file_no=20220304&year_id=2022&quarter_id=3&falg=1紫海胆；脂质；水酶法；理化性质；脂肪酸；脂质组成；熔融；结晶heliocidaris crassispina; lipid; enzyme-assisted aqueous method; physicochemical property; fatty acid; lipid composition; melting; crystallization |
| spellingShingle | 潘南1，江婷婷2，陈晓婷1，徐清云3，吴靖娜4,5，蔡水淋1,6，廖登远1，刘智禹1 PAN Nan1， JIANG Tingting2， CHEN Xiaoting1， XU Qingyun3， WU Jingna4,5， CAI Shuilin1,6， LIAO Dengyuan1， LIU Zhiyu1 水酶法提取紫海胆性腺脂质的工艺优化 及提取方法对其品质的影响Process optimization of lipid extraction from the gonad of Heliocidaris crassispina via enzyme-assisted aqueous method and effect of extraction methods on its quality Zhongguo youzhi 紫海胆；脂质；水酶法；理化性质；脂肪酸；脂质组成；熔融；结晶 heliocidaris crassispina; lipid; enzyme-assisted aqueous method; physicochemical property; fatty acid; lipid composition; melting; crystallization |
| title | 水酶法提取紫海胆性腺脂质的工艺优化 及提取方法对其品质的影响Process optimization of lipid extraction from the gonad of Heliocidaris crassispina via enzyme-assisted aqueous method and effect of extraction methods on its quality |
| title_full | 水酶法提取紫海胆性腺脂质的工艺优化 及提取方法对其品质的影响Process optimization of lipid extraction from the gonad of Heliocidaris crassispina via enzyme-assisted aqueous method and effect of extraction methods on its quality |
| title_fullStr | 水酶法提取紫海胆性腺脂质的工艺优化 及提取方法对其品质的影响Process optimization of lipid extraction from the gonad of Heliocidaris crassispina via enzyme-assisted aqueous method and effect of extraction methods on its quality |
| title_full_unstemmed | 水酶法提取紫海胆性腺脂质的工艺优化 及提取方法对其品质的影响Process optimization of lipid extraction from the gonad of Heliocidaris crassispina via enzyme-assisted aqueous method and effect of extraction methods on its quality |
| title_short | 水酶法提取紫海胆性腺脂质的工艺优化 及提取方法对其品质的影响Process optimization of lipid extraction from the gonad of Heliocidaris crassispina via enzyme-assisted aqueous method and effect of extraction methods on its quality |
| title_sort | 水酶法提取紫海胆性腺脂质的工艺优化 及提取方法对其品质的影响process optimization of lipid extraction from the gonad of heliocidaris crassispina via enzyme assisted aqueous method and effect of extraction methods on its quality |
| topic | 紫海胆；脂质；水酶法；理化性质；脂肪酸；脂质组成；熔融；结晶 heliocidaris crassispina; lipid; enzyme-assisted aqueous method; physicochemical property; fatty acid; lipid composition; melting; crystallization |
| url | http://tg.chinaoils.cn/ch/reader/create_pdf.aspx?file_no=20220304&year_id=2022&quarter_id=3&falg=1 |
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