Isolation, purification and identification of polysaccharide of Ficus carica(无花果多糖的分离、纯化和鉴定)
经脱脂、沸水抽提、乙醇沉淀、酶-Sevag法脱蛋白得到粗多糖，再经DEAE-Sepharose fast flow阴离子交换层析和Sephadex G-200葡聚糖凝胶柱层析得到纯化的无花果多糖(FCPS)，用红外光谱和紫外光谱分析鉴定该多糖.红外光谱分析具有典型的多糖特征吸收峰，紫外光谱分析未见蛋白质(280 nm)与核酸(260 nm)的特征吸收峰. 结果表明，所提取的无花果多糖为单一组分的多糖....
| Main Authors: | , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Zhejiang University Press
2004-03-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
| Subjects: | |
| Online Access: | https://doi.org/zjup/1008-9497.2004.31.2.177-179 |