Tyrosinase Depletion Prevents the Maturation of Melanosomes in the Mouse Hair Follicle.
The mechanisms that lead to variation in human skin and hair color are not fully understood. To better understand the molecular control of skin and hair color variation, we modulated the expression of Tyrosinase (Tyr), which controls the rate-limiting step of melanogenesis, by expressing a single-co...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4664286?pdf=render |
| _version_ | 1818949573839683584 |
|---|---|
| author | Elyse K Paterson Thomas J Fielder Grant R MacGregor Shosuke Ito Kazumasa Wakamatsu Daniel L Gillen Victoria Eby Raymond E Boissy Anand K Ganesan |
| author_facet | Elyse K Paterson Thomas J Fielder Grant R MacGregor Shosuke Ito Kazumasa Wakamatsu Daniel L Gillen Victoria Eby Raymond E Boissy Anand K Ganesan |
| author_sort | Elyse K Paterson |
| collection | DOAJ |
| description | The mechanisms that lead to variation in human skin and hair color are not fully understood. To better understand the molecular control of skin and hair color variation, we modulated the expression of Tyrosinase (Tyr), which controls the rate-limiting step of melanogenesis, by expressing a single-copy, tetracycline-inducible shRNA against Tyr in mice. Moderate depletion of TYR was sufficient to alter the appearance of the mouse coat in black, agouti, and yellow coat color backgrounds, even though TYR depletion did not significantly inhibit accumulation of melanin within the mouse hair. Ultra-structural studies revealed that the reduction of Tyr inhibited the accumulation of terminal melanosomes, and inhibited the expression of genes that regulate melanogenesis. These results indicate that color in skin and hair is determined not only by the total amount of melanin within the hair, but also by the relative accumulation of mature melanosomes. |
| first_indexed | 2024-12-20T09:04:51Z |
| format | Article |
| id | doaj.art-f686141cdd10446dbe864532116f42bf |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T09:04:51Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f686141cdd10446dbe864532116f42bf2022-12-21T19:45:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011011e014370210.1371/journal.pone.0143702Tyrosinase Depletion Prevents the Maturation of Melanosomes in the Mouse Hair Follicle.Elyse K PatersonThomas J FielderGrant R MacGregorShosuke ItoKazumasa WakamatsuDaniel L GillenVictoria EbyRaymond E BoissyAnand K GanesanThe mechanisms that lead to variation in human skin and hair color are not fully understood. To better understand the molecular control of skin and hair color variation, we modulated the expression of Tyrosinase (Tyr), which controls the rate-limiting step of melanogenesis, by expressing a single-copy, tetracycline-inducible shRNA against Tyr in mice. Moderate depletion of TYR was sufficient to alter the appearance of the mouse coat in black, agouti, and yellow coat color backgrounds, even though TYR depletion did not significantly inhibit accumulation of melanin within the mouse hair. Ultra-structural studies revealed that the reduction of Tyr inhibited the accumulation of terminal melanosomes, and inhibited the expression of genes that regulate melanogenesis. These results indicate that color in skin and hair is determined not only by the total amount of melanin within the hair, but also by the relative accumulation of mature melanosomes.http://europepmc.org/articles/PMC4664286?pdf=render |
| spellingShingle | Elyse K Paterson Thomas J Fielder Grant R MacGregor Shosuke Ito Kazumasa Wakamatsu Daniel L Gillen Victoria Eby Raymond E Boissy Anand K Ganesan Tyrosinase Depletion Prevents the Maturation of Melanosomes in the Mouse Hair Follicle. PLoS ONE |
| title | Tyrosinase Depletion Prevents the Maturation of Melanosomes in the Mouse Hair Follicle. |
| title_full | Tyrosinase Depletion Prevents the Maturation of Melanosomes in the Mouse Hair Follicle. |
| title_fullStr | Tyrosinase Depletion Prevents the Maturation of Melanosomes in the Mouse Hair Follicle. |
| title_full_unstemmed | Tyrosinase Depletion Prevents the Maturation of Melanosomes in the Mouse Hair Follicle. |
| title_short | Tyrosinase Depletion Prevents the Maturation of Melanosomes in the Mouse Hair Follicle. |
| title_sort | tyrosinase depletion prevents the maturation of melanosomes in the mouse hair follicle |
| url | http://europepmc.org/articles/PMC4664286?pdf=render |
| work_keys_str_mv | AT elysekpaterson tyrosinasedepletionpreventsthematurationofmelanosomesinthemousehairfollicle AT thomasjfielder tyrosinasedepletionpreventsthematurationofmelanosomesinthemousehairfollicle AT grantrmacgregor tyrosinasedepletionpreventsthematurationofmelanosomesinthemousehairfollicle AT shosukeito tyrosinasedepletionpreventsthematurationofmelanosomesinthemousehairfollicle AT kazumasawakamatsu tyrosinasedepletionpreventsthematurationofmelanosomesinthemousehairfollicle AT daniellgillen tyrosinasedepletionpreventsthematurationofmelanosomesinthemousehairfollicle AT victoriaeby tyrosinasedepletionpreventsthematurationofmelanosomesinthemousehairfollicle AT raymondeboissy tyrosinasedepletionpreventsthematurationofmelanosomesinthemousehairfollicle AT anandkganesan tyrosinasedepletionpreventsthematurationofmelanosomesinthemousehairfollicle |