Single-cell spatial architectures associated with clinical outcome in head and neck squamous cell carcinoma
Abstract There is increasing evidence that the spatial organization of cells within the tumor-immune microenvironment (TiME) of solid tumors influences survival and response to therapy in numerous cancer types. Here, we report results and demonstrate the applicability of quantitative single-cell spa...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-02-01
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| Series: | npj Precision Oncology |
| Online Access: | https://doi.org/10.1038/s41698-022-00253-z |
| _version_ | 1797642748982984704 |
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| author | Katie E. Blise Shamilene Sivagnanam Grace L. Banik Lisa M. Coussens Jeremy Goecks |
| author_facet | Katie E. Blise Shamilene Sivagnanam Grace L. Banik Lisa M. Coussens Jeremy Goecks |
| author_sort | Katie E. Blise |
| collection | DOAJ |
| description | Abstract There is increasing evidence that the spatial organization of cells within the tumor-immune microenvironment (TiME) of solid tumors influences survival and response to therapy in numerous cancer types. Here, we report results and demonstrate the applicability of quantitative single-cell spatial proteomics analyses in the TiME of primary and recurrent human papillomavirus (HPV)-negative head and neck squamous cell carcinoma (HNSCC) tumors. Single-cell compositions of a nine patient, primary and recurrent (n = 18), HNSCC cohort is presented, followed by deeper investigation into the spatial architecture of the TiME and its relationship with clinical variables and progression free survival (PFS). Multiple spatial algorithms were used to quantify the spatial landscapes of immune cells within TiMEs and demonstrate that neoplastic tumor-immune cell spatial compartmentalization, rather than mixing, is associated with longer PFS. Mesenchymal (αSMA+) cellular neighborhoods describe distinct immune landscapes associated with neoplastic tumor-immune compartmentalization and improved patient outcomes. Results from this investigation are concordant with studies in other tumor types, suggesting that trends in TiME cellular heterogeneity and spatial organization may be shared across cancers and may provide prognostic value in multiple cancer types. |
| first_indexed | 2024-03-11T14:04:46Z |
| format | Article |
| id | doaj.art-f688fa4c36d04692b5dff26739d4cdf0 |
| institution | Directory Open Access Journal |
| issn | 2397-768X |
| language | English |
| last_indexed | 2024-03-11T14:04:46Z |
| publishDate | 2022-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Precision Oncology |
| spelling | doaj.art-f688fa4c36d04692b5dff26739d4cdf02023-11-02T02:53:27ZengNature Portfolionpj Precision Oncology2397-768X2022-02-016111410.1038/s41698-022-00253-zSingle-cell spatial architectures associated with clinical outcome in head and neck squamous cell carcinomaKatie E. Blise0Shamilene Sivagnanam1Grace L. Banik2Lisa M. Coussens3Jeremy Goecks4Department of Biomedical Engineering, Oregon Health & Science UniversityThe Knight Cancer Institute, Oregon Health & Science UniversityDepartment of Cell, Developmental & Cancer Biology, Oregon Health & Science UniversityThe Knight Cancer Institute, Oregon Health & Science UniversityDepartment of Biomedical Engineering, Oregon Health & Science UniversityAbstract There is increasing evidence that the spatial organization of cells within the tumor-immune microenvironment (TiME) of solid tumors influences survival and response to therapy in numerous cancer types. Here, we report results and demonstrate the applicability of quantitative single-cell spatial proteomics analyses in the TiME of primary and recurrent human papillomavirus (HPV)-negative head and neck squamous cell carcinoma (HNSCC) tumors. Single-cell compositions of a nine patient, primary and recurrent (n = 18), HNSCC cohort is presented, followed by deeper investigation into the spatial architecture of the TiME and its relationship with clinical variables and progression free survival (PFS). Multiple spatial algorithms were used to quantify the spatial landscapes of immune cells within TiMEs and demonstrate that neoplastic tumor-immune cell spatial compartmentalization, rather than mixing, is associated with longer PFS. Mesenchymal (αSMA+) cellular neighborhoods describe distinct immune landscapes associated with neoplastic tumor-immune compartmentalization and improved patient outcomes. Results from this investigation are concordant with studies in other tumor types, suggesting that trends in TiME cellular heterogeneity and spatial organization may be shared across cancers and may provide prognostic value in multiple cancer types.https://doi.org/10.1038/s41698-022-00253-z |
| spellingShingle | Katie E. Blise Shamilene Sivagnanam Grace L. Banik Lisa M. Coussens Jeremy Goecks Single-cell spatial architectures associated with clinical outcome in head and neck squamous cell carcinoma npj Precision Oncology |
| title | Single-cell spatial architectures associated with clinical outcome in head and neck squamous cell carcinoma |
| title_full | Single-cell spatial architectures associated with clinical outcome in head and neck squamous cell carcinoma |
| title_fullStr | Single-cell spatial architectures associated with clinical outcome in head and neck squamous cell carcinoma |
| title_full_unstemmed | Single-cell spatial architectures associated with clinical outcome in head and neck squamous cell carcinoma |
| title_short | Single-cell spatial architectures associated with clinical outcome in head and neck squamous cell carcinoma |
| title_sort | single cell spatial architectures associated with clinical outcome in head and neck squamous cell carcinoma |
| url | https://doi.org/10.1038/s41698-022-00253-z |
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