Detection of SARS-CoV-2 RNA by direct RT-qPCR on nasopharyngeal specimens without extraction of viral RNA.

Detection of SARS-CoV-2 RNA by direct RT-qPCR on nasopharyngeal specimens without extraction of viral RNA.

To circumvent the limited availability of RNA extraction reagents, we aimed to develop a protocol for direct RT-qPCR to detect SARS-CoV-2 in nasopharyngeal swabs without RNA extraction. Nasopharyngeal specimens positive for SARS-CoV-2 and other coronaviruses collected in universal viral transport (U...

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Bibliographic Details
Main Authors: Mohammad Rubayet Hasan, Faheem Mirza, Hamad Al-Hail, Sathyavathi Sundararaju, Thabisile Xaba, Muhammad Iqbal, Hashim Alhussain, Hadi Mohamad Yassine, Andres Perez-Lopez, Patrick Tang
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2020-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0236564

Internet

https://doi.org/10.1371/journal.pone.0236564

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