Functionalized graphene grids with various charges for single-particle cryo-EM

Functionalized graphene grids with various charges for single-particle cryo-EM

Air-water interface and preferential orientation problems are crucial challenges in cryo-EM specimen preparation. Here, the authors utilize graphene-coated EM grids functionalized by salts with various electrostatic properties, successfully overcoming preferred orientation.

Bibliographic Details
Main Authors: Ye Lu, Nan Liu, Yongbo Liu, Liming Zheng, Junhao Yang, Jia Wang, Xia Jia, Qinru Zi, Hailin Peng, Yu Rao, Hong-Wei Wang
Format: Article
Language:English
Published: Nature Portfolio 2022-11-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-022-34579-w

Internet

https://doi.org/10.1038/s41467-022-34579-w

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