Electrophoresis of amplicons is a better method to understand the performance of loop-mediated isothermal amplification assay for screening the presence of Escherichia coli in water

Electrophoresis of amplicons is a better method to understand the performance of loop-mediated isothermal amplification assay for screening the presence of Escherichia coli in water

LAMP assay is widely used for detecting pathogens. We observed that the conventional and gradient polymerase chain reaction (PCR) could not detect the extracted Escherichia coli DNA; real-time PCR was able to detect up to a certain limit (10−8 bacterial dilution). At the same time, the LAMP assay co...

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Bibliographic Details
Main Authors: Sumanpreet Kaur, Deepak Kumar, Sheemona Chowdhary, Rajasri Bhattacharyya, Dibyajyoti Banerjee
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2023-01-01
Series:Indian Journal of Public Health
Subjects:
dna
dyes
escherichia coli
electrophoresis
lamp assay
water contamination
Online Access:http://www.ijph.in/article.asp?issn=0019-557X;year=2023;volume=67;issue=4;spage=530;epage=532;aulast=Kaur

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http://www.ijph.in/article.asp?issn=0019-557X;year=2023;volume=67;issue=4;spage=530;epage=532;aulast=Kaur

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