Development of a gRNA Expression and Processing Platform for Efficient CRISPR-Cas9-Based Gene Editing and Gene Silencing in Candida tropicalis

Development of a gRNA Expression and Processing Platform for Efficient CRISPR-Cas9-Based Gene Editing and Gene Silencing in Candida tropicalis

ABSTRACT Candida tropicalis, a nonmodel diploid microbe, has been applied in industry as a chassis cell. Metabolic engineering of C. tropicalis is challenging due to a lack of gene editing and regulation tools. Here, we report a tRNA:guide RNA (gRNA) platform for boosting gene editing and silencing...

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Bibliographic Details
Main Authors: Yujie Li, Lihua Zhang, Haiquan Yang, Yuanyuan Xia, Liming Liu, Xianzhong Chen, Wei Shen
Format: Article
Language:English
Published: American Society for Microbiology 2022-06-01
Series:Microbiology Spectrum
Subjects:
Candida tropicalis
CRISPR interference system
RNA Pol III promoter
genome editing
gRNA expression element
Online Access:https://journals.asm.org/doi/10.1128/spectrum.00059-22

Internet

https://journals.asm.org/doi/10.1128/spectrum.00059-22

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